PAPILLOMAVIRIDAE

Question 1

General

Answer 1

Family-Papillomaviridae-

One Genus=Papilloma Virus
papilla-wart, oma-tumour

Question 2

Morphology

Answer 2

Non-enveloped
Icosahedral capsid
dsDNA-(Circular)

Superhelical; ~ 55 nm

Question 3

Resistance

Answer 3

Strong
With strands
(56°C, 30 min; pH 3, lipid solvents)

Question 4

Replication

Answer 4

In the nucleus by cellular transcriptase

Question 5

Biological properties

Answer 5

• Propagate in keratinized cells (skin cells)
• Stenoxenous
• Oncogenic
• Latent
  
  • Can hide easily from the immune system
• Transmitted by: contact/sex

Question 6

Antigenic properties

Answer 6

Good

Question 7

Diagnosis

Answer 7

Clinical signs
IF
EM
NA hybridisation

Question 8

Papylomavirus genus

Answer 8

1. Equine papilomatosis - Head, nose
2. Bovine papilomatosis - neck, head, back
3. Horse sarcoid - tumours around eye
4. Canine oral papilomatosis - oral mucosa

Human:

1. Skin papilomas - verruca vulgaris
2. Genital papilomas - STD
3. Cervical cancer (16 and 18)

Question 9

Warts=

Answer 9

Papillomas:

Benignant tumors – pre-malignant status