(Part 1) 1.18 Specimen management

Question 1

Specimens should be collected during ___________ of an illness and before antimicrobials, antifungals, or antiviral medications are administered, if possible

Answer 1

acute phase/ early stage

Question 2

within how many mins/hrs should sterile body fluids such as CSF and other body fluids should be transported to the lab?

Answer 2

15 mins

Question 3

within how many mins/hrs should stool and urine be transported to the lab?

Answer 3

2 hrs

Question 4

All specimen containers should be leak-proof, and the specimens should be transported within sealable leak-proof, plastic bags with a separate section for paperwork

T or F

Answer 4

T

Question 5

Specimen integrity includes?

Answer 5

Correct modes of COLLECTION

HANDLING of specimen

PRESERVATION

time of TRANSPORT

Question 6

Specimen that should be stored in 4 C

Answer 6

Urine
Stool
Sputum
Swab
Catheter tips

(youSSS see) USSSC

Question 7

Specimens that should be stored in 25 C

Answer 7

CSF
Body fluids (Serous fluids)
IV catheters
Abscess
Gastric aspirate
Genital specimen
Throat/Nasopharyngeal

Question 8

Specimens that should be stored in 35 C

Answer 8

CSF (Hold only for 6 hrs)

Question 9

Blood is drawn at time of _______ episode

Answer 9

Febrile

Question 10

How many sample of blood should be taken?

Answer 10

2-3 samples

Question 11

Blood Volume for adults and pedia?

Answer 11

Adults: 20 mL
Pediatric: 1-20mL

Question 12

Anticoagulant used in blood?

This inhibits phagocytosis (Neutralizes bactericidal effect in human serum)

Answer 12

0.025% SPS (Sodium Polyanethol Sulfonate)

Question 13

Routine blood culture is done every?

Answer 13

5-7 days

Question 14

How sputum is collected?

Answer 14

Deep cough

Question 15

how to determine the sputum suitability fr gram staining?

Answer 15

<10 epithelial cells / LPF
>25 PMNS / LPF

Question 16

> 10 epithelial cells / LPF
<25 PMNS / LPF

is this suitable?

Answer 16

no, the specimen is saliva

Question 17

How to collect urine?

Answer 17

Midstream catch

Question 18

How to collect urine if the specimen requires anaerobically?

Answer 18

Suprapubic Urine

Question 19

How to collect catheterized urine specimens?

Answer 19

First 15mL discard then the remaining collect

Question 20

Preservative used for urine?

Answer 20

Boric Acid

Question 21

Major cause of ascending UTI?

Answer 21

Escherichia coli

Question 22

What bacteria is to be expected if stool samples is received?

Answer 22

Salmonella and Shigella, Campylobacter

Question 23

What is the transport media used for stool and rectal swab if the transportation exceeds 1 hr?

Answer 23

Cary Blair medium

Question 24

How to collect rectal swab?

Answer 24

1-1.5 cm past anal sphincter

Question 25

What tube # is being collected in CSF?

Answer 25

Tube 2

Question 26

How to store CSF?

Answer 26

35C for 6hrs

Question 27

If only one tube of CSF is received, what is the chronological order of lab to receive csf specimen?

Answer 27

1st Microbiology 2nd Hematology 3rd Chemistry and Serology

Question 28

What specimen is used for routine culture of Group A Streptococcus

Answer 28

Throat/Pharynx

Question 29

Specimen of choice for Bordetella pertusis?

Answer 29

Nasopharynx

Question 30

How to collect nasopharynx?

Answer 30

Similar to collecting COVID 19 specimen swab and rotate for 5 secs in nasopharynx

Question 31

NOTE: (Superficial abscess) * Wipe area with sterile saline or 70% alcohol * Aspirate if possible, swab along leading edge of wound

Question 32

Most important in specimen labeling

Answer 32

Complete patient's name

Question 33

incubation Temp for Fungi?

Answer 33

28 - 30C

Question 34

Incubation temp for most bacteria, viruses and acid fast bacillus?

Answer 34

35-37C 35C more optimum bacterial growth

Question 35

21% oxygen 0.03% CO2

Answer 35

Aerobes

Question 36

5-10% CO2 15% Oxygen

Answer 36

Capnophiles

Question 37

5-10% Oxygen 8-10% CO2

Answer 37

Microaerophiles

Question 38

* Do not require oxygen * 5% to 10% hydrogen * 5% to 10% CO2 * 80% to 90% nitrogen * 0% O2

Answer 38

Anaerobes