(Part 2) 2.23.2 Mycobacterium Detection, tests, and culture Flashcards by Miguel Acosta

Q

Example of types of acid fast stains?

A

Ziehl Neelsen
Kinyuon
Auramine-Rhodamine

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Q

Before a smear is reported as negative, it should be examined carefully by scanning at least ________ OIFs

a. 200
b. 250
c. 300
d. 350

A

c

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Q

Number of AFB seen: 0

Report?

a. No AFB seen
b. Indeterminate
c. 1+
d. 2+
e. 3+
f. 4+

A

a

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Q

Number of AFB seen: 1-9/10 fields

Report?

a. No AFB seen
b. Indeterminate
c. 1+
d. 2+
e. 3+
f. 4+

A

d

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Q

Number of AFB seen: 1-9/100 fields

Report?

a. No AFB seen
b. Indeterminate
c. 1+
d. 2+
e. 3+
f. 4+

A

c

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Q

Number of AFB seen: 1-9/ fields

Report?

a. No AFB seen
b. Indeterminate
c. 1+
d. 2+
e. 3+
f. 4+

A

e

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Q

Number of AFB seen: 1-2/300 fields

Report?

a. No AFB seen
b. Indeterminate
c. 1+
d. 2+
e. 3+
f. 4+

A

b

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Q

Number of AFB seen: 9+/fields

Report?

a. No AFB seen
b. Indeterminate
c. 1+
d. 2+
e. 3+
f. 4+

A

f

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Q

Cell wall component of MTB

a. Lipopolysaccharide
b. Peptidoglycan
c. Liparabinomannan
d. Mycolic acid

A

c

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Q

Lipoarabinomannan can be seen in ELISA or Lateral flow assays

T or F

A

T

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Q

Culture media for MTB Agar base?

a. MiddleBrook7H10 and 7H11
b. Lowenstein Jensen
c. Petragani
d. BAP

A

a

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Q

Agar-based media can grow MTB within 18-24 days

T or F

A

F

10-12 days

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Q

Culture media for MTB Egg base?

a. MiddleBrook7H10 and 7H11
b. Lowenstein Jensen
c. Petragani
d. BAP

A

b and c

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Q

Egg-based media can grow MTB within 18-24 days

T or F

A

T

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Q

Example of Liquid media for MTB are

BACTEC 12B medium
Middlebrook 7H9 broth
Dubos Tween albumin
Septi-Chek AFB

Which does not belong

A

NOne

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Q

Cultures for MTB are incubated at 35 C in the light in a atm of 5% to 10% CO2 and high humidity

T or F

A

F

In the Dark

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Q

Cultures of MTB should Examine weekly for growth; most isolated appear between 3 and 6 weeks

T or F

A

T

Q

All mycobacterium spp produce niacin but M. tuberculosis accumulates the largest amount

T or F

A

T

Q

Positive and Negative result for Niacin?

A

(+) Yellow
(-) No color change

Q

Helpful in identifying M. tuberculosis, M. kansasii, M.szulgai, M. fortuitum

a. Niacin
b. Nitrate
c. Urease
d. Tween 80

A

b

Q

Positive and Negative result for Nitrate

A

(+) Pink / Red
(-) No color change

Q

Helpgul in identifying M. fortuitum, M. kansasii, M. szulgai, M. flavescens

a. Niacin
b. Nitrate
c. Urease
d. Tween 80

A

c

Q

Positive and Negative result for Urease

A

(+) Pink or Red (Magenta)
(-) No color change (Yellow)

Q

a. Niacin
b. Nitrate
c. Urease
d. Tween 80

A

End product of Tween 80

Lipase

Helpful in identifying slow growing scotochromogens and nonphotochromogens a. Niacin b. Nitrate c. Urease d. Tween 80

d

Positive and Negative result for Tween 80

(+) Pink and Red (-) No color change

What is the temp for heat stable catalase test? a. 42C b. 69C c. 43C d. 68C

68 C

Positive indicator for Catalse

Bubbles

based on relative activity of the enzyme as determine by the height of a column of bubbles of oxygen form a. Heat stable Catalase b. Semiqualitative catalase c. Semiquantitative catalase d. Quantitative catalase

c

Positive and negative for Semiquantitative catalase

(+) >45 mm (-) <45mm

Spp of NTM that reduce potassium tellurite

M. avium complex

positive and negative result for Tellurite?

(+) Smooth, fine, black precipitate (-) Gray clumps

This enzyme is present in most mycobacteria a. Aminase b. Sulfatase c. Arysulfatase d. Aminoladase

c

3 day test that is useful for identifying the potentially pathogenic rapid growers M. fortuitum, M. chelonae a. Tellurite b. 5% NaCl c. Arysulfatase d. Thiophene-2-Carboxylic acid hydrazide

c

Positive and negative for Arysulfatase?

(+) Pink and red (-) No color change

Rapid grower that can tolerate 5% NaCl?

M. fortuitum

Positive and negative for 5% NaCl

(+) Substantial growth (-) Little or no growth

It used to distinguish what M. bovis from M. tuberculosis a. Tellurite b. 5% NaCl c. Arysulfatase d. Thiophene-2-Carboxylic acid hydrazide

d

Thiophene-2-carboxylic acid hydrazide (10 mg/mL) Positive and Negative

(+) No growth (-) Growth