(Part 2) 2.1.1 Test and Media for Staph and Micrococcus Flashcards
Staph and Micrococcus are Catalase Positive
T or F
T
Only Staph can grow in an aerobic environment while micrococcus not
T or F
F
Micrococcus can grow in an Anaerobic environment
T or F
F
Obligate aerobe
Staph can oxidize Glucose
T or F
F
Ferment
Micrococcus can’t ferment glucose
T or F
T
Bacteria that have a result of Blue-Purple in Microdase test
Micrococcus
Positive in benzidine?
Staph or micro
Micro
Sus in Lysostaphin?
Staph or micro
Staph
Res in Taxo A
Staph or micro
Staph
Sus in Lysostaphin
Staph or Micro
Staph
What is the reagent used in Catalase test for Staph and micrococcus
3% Hydrogen Peroxide (H2O2)
What is the reagent for Microdase test / Modified oxidase test
Tetramethyl-Phenylenediamene-Dihydrochloride in a DimethylSulfoxide
(TMPD in a DMSO)
What is Positive result for Catalase test?
Vigorous bubbling
What is the Positive result for Microdase Test
BLue-Purple/BLue
What is the principle for Catalase test?
Catalase enzyme hydrolyzes H2O2 in to Water (H2O) and Oxygen (O2) which result to bubbling
What is the Medium for DNase test?
DNA methyl green complex
What is the result for DNase test?
Clearing of Medium
What is the principle for DNase test?
The ability of the bacteria to hydrolyzes DNA medium complex which results to clearing
SBA and CHOC agar can be used to grow Staph?
T or F
T
What are the selective medium for Staph?
PEA and CNA agar
What is the Selective-Differential media for Staph?
Mannitol salt agar
What is the inhibitor for MSA?
7.5% NaCl
What is the component of MSA that differentiates Staph?
Mannitol
What is the pH indicator for MSA/
Phenol Red
