(Part 2) 2.22.1 Anaerobes media and incubations

Question 1

Anaerobes lacks superoxide dismutase and catalase which are enzymes required to breakdown nitrogen

T or F

Answer 1

F

Not N its oxygen

Question 2

Prevotella

O. anaerobes / Aerotolerant anaerobes

Answer 2

O anaerobes

Question 3

Actinomyces

O. anaerobes / Aerotolerant anaerobes

Answer 3

Aerotolerant

Question 4

Bifidoacterium

O. anaerobes / Aerotolerant anaerobes

Answer 4

Aero

Question 5

Fusobacterium

O. anaerobes / Aerotolerant anaerobes

Answer 5

Obligate

Question 6

Bacteroides

O. anaerobes / Aerotolerant anaerobes

Answer 6

Obligaate

Question 7

Clostridium

O. anaerobes / Aerotolerant anaerobes

Answer 7

Aero

Question 8

Best specimen for anaerobes are Tissue biopsy and Needle Aspiration

T or F

Answer 8

T

Question 9

Swabs are recommended for Anaerobes sample collection

T or F

Answer 9

F

Question 10

Anaerobic media contains

Hemin
Blood
Vitamin E

T or F

Answer 10

F

Vitamine K

Question 11

Anaerobic media that provides a source of CO2

Answer 11

Sodium Bicarbonate

Question 12

Purpose of reducing agents?

Answer 12

Absorb oxygen

Question 13

is used for isolation of all anaerobic organism including obligate and facultative anaerobes.

1. Anaerobic blood agar
2. Anaerobic PEA blood agar
3. Anaerobic kanamycin-vancomycin blood agar
4. Anaerobic paromomycin-vancomycin laked blood agar (PVLB)
5. Thioglycollate
6. Bacteroides bile-esculin (BBE)

Answer 13

1

Question 14

The antibiotic
vancomycin will inhibit facultative and obligate gram positive anaerobes while kanamycin inhibits the growth of facultative gram negative rods. It is use to isolate gram negative anaerobes like bacteroides, prevotella, and some fusobacterium

1. Anaerobic blood agar
2. Anaerobic PEA blood agar
3. Anaerobic kanamycin-vancomycin blood agar
4. Anaerobic paromomycin-vancomycin laked blood agar (PVLB)
5. Thioglycollate
6. Bacteroides bile-esculin (BBE)

Answer 14

3

Question 15

can inhibit the swarming of proteus and also inhibits the growth of facultative anaerobes such as the members of enterobacteriaceae. We can isolate gram positive and most gram negative obligate anaerobes

1. Anaerobic blood agar
2. Anaerobic PEA blood agar
3. Anaerobic kanamycin-vancomycin blood agar
4. Anaerobic paromomycin-vancomycin laked blood agar (PVLB)
5. Thioglycollate
6. Bacteroides bile-esculin (BBE)

Answer 15

2

Question 16

Used to detect prevotella due to pigment production

1. Anaerobic blood agar
2. Anaerobic PEA blood agar
3. Anaerobic kanamycin-vancomycin blood agar
4. Anaerobic paromomycin-vancomycin laked blood agar (PVLB)
5. Thioglycollate
6. Bacteroides bile-esculin (BBE)

Answer 16

4

Question 17

detects organism that has the ability to grow in 20% bile and be able to hydrolyze esculin. Positive result for Esculin hydrolysis is brownblack color

1. Anaerobic blood agar
2. Anaerobic PEA blood agar
3. Anaerobic kanamycin-vancomycin blood agar
4. Anaerobic paromomycin-vancomycin laked blood agar (PVLB)
5. Thioglycollate
6. Bacteroides bile-esculin (BBE)

Answer 17

6

Question 18

is enriched broth with supplemented hemin and vitamin. It is use as a supplement to plating media or slow growing bacteria.

1. Anaerobic blood agar
2. Anaerobic PEA blood agar
3. Anaerobic kanamycin-vancomycin blood agar
4. Anaerobic paromomycin-vancomycin laked blood agar (PVLB)
5. Thioglycollate
6. Bacteroides bile-esculin (BBE)

Answer 18

5

Question 19

Used to demonstrate lecithinase activity

a. Egg yolk agar
b. Bile esculin
c. Thioglycollate

Answer 19

a

Question 20

Used to grow obligate and aerotolerant anaerobes

a. Egg yolk agar
b. Bile esculin
c. Thioglycollate

Answer 20

c

Question 21

Used to grow bacteroides

a. Egg yolk agar
b. Bile esculin
c. Thioglycollate

Answer 21

b

Question 22

Oxygen is removed from the system through reaction with hydrogen,
which has been added externally to the system. Reaction takes place in
the presence of palladium catalyst.

A. anaerobic Jar method
b. Evacuation-replacement system
c. Glove-Box method
d. Disposable anaerobic bags

Answer 22

a

Question 23

What is the indicator for anaerobic jar method?

a. Methylene blue strips
b. Resazurin dye

Answer 23

a

Question 24

In methylene blue strips, Color of methylene blue strip if oxygen is gone?

Answer 24

White

Question 25

In methylene blue strips, Color of methylene blue strip if oxygen remain?

Answer 25

Blue

Question 26

Indicator for Anaerobic jar method where the color of the indicator is pink (O2 present), white (absent) a. Methylene blue strips b. Resazurin dye

Answer 26

b

Question 27

The air in the jar is removed and replaced with a mixture of gas containing a. 85% N2, 5% H2, and 10% CO2. b. 85% N2, 5% H2, and 5% CO2. c. 65% N2, 10% H2, and 5% CO2. d. 85% N2, 10% H2, and 5% CO2.

Answer 27

d

Question 28

The air in the jar is removed and replaced with a mixture of gas containing 85% N2, 10% H2, and 5% CO2. A. anaerobic Jar method b. Evacuation-replacement system c. Glove-Box method d. Disposable anaerobic bags

Answer 28

b

Question 29

Redox indicator for Evacuation-replacement system?

Answer 29

Methylene blue strip

Question 30

Self-contained anaerobic environment of 85% N2, 10% H2, and 5% CO2 is provided through external gas sources Useful for large laboratories that handle large volume of anaerobic specimens A. anaerobic Jar method b. Evacuation-replacement system c. Glove-Box method d. Disposable anaerobic bags

Answer 30

c

Question 31

Plastic bags with a H2- CO2 gas generator that is released on addition of water Can hold 1-3 petri dish) A. anaerobic Jar method b. Evacuation-replacement system c. Glove-Box method d. Disposable anaerobic bags

Answer 31

d

Question 32

Alternative of Anaerobic Jar method?

Answer 32

Candle jar

Question 33

This an anaerobic incubation sysem that is useful for large laboratorus that handles large volume of anaerobic samples

Answer 33

Anaerobic chambers