(Part 2) 2.2.3 Strep Media and Test

Question 1

Which of the following cant grow Strep spp.?

SBA
CHOC
PEA
CNA

Answer 1

None

Question 2

Abiotrophia and Granulicatella cannot grow in SBA

T or F

Answer 2

T

Question 3

Abiotrophia and Granulicatella requires what vitamin to be grown?

Answer 3

Vitamin B6

Question 4

Used for Isolating GAS from Throat Swabs? (Full name)

Answer 4

5% Sheep blood Agar
w/
Trimetophrim-Sulfamethoxazole (SXT)

Question 5

LIM broth isolates what strep?

Answer 5

Group B strep

Question 6

LIM broth is a Todd-Hewitt broth that contains?

Answer 6

CNA (Colistin Nalidixic acid)

Question 7

A selective - Differential based on esculing hydrolysis that isolates enterococci?

Answer 7

Enterococcosel agar

Question 8

What is the Sus ZOI for Taxo P?

What is Taxo P?

Answer 8

> 14mm

Optochin

Question 9

What is the Sus ZOI for Taxo A

What is Taxo A?

Answer 9

> 10mm

Bacitracin

Question 10

What is the reagent of PYR?

Answer 10

Pyrolidonyl-Naphthylamide

Question 11

What is the positive indicator for PYR?

Answer 11

Bright Red

Question 12

What is the meaning of CAMP?

Answer 12

Christie-Atkins-Munch-Peterson

Question 13

What is the Streaked organism (Control) used for CAMP [Vertical]?

Answer 13

S. aurues

Question 14

What is the result for CAMP?

Answer 14

Arrowhead

Question 15

CAMP is used to determine what strep?

Answer 15

S. agalactiae

Question 16

S. aureus act as a source of ______ in CAMP

Answer 16

Beta lysin

Enhance Beta lysin for Group B

Question 17

What media is used for CAMP?

Answer 17

BAP

Question 18

What is the reagent for Hippurate hydolysis

Answer 18

Hippuric acid / Benzoic acid

Question 19

What happens to hippuric acid when is being hydrolysed

Answer 19

Hippric acid -> glycine

Question 20

Positive indicator for Hippurate hydrolysis

Answer 20

Purple

Question 21

What strep is positive for Hippurate hydrolysis?

Answer 21

Strep agalactiae

Question 22

What is the inhibitor used in Bile esculing hydrolysis?

Answer 22

40% bile

Question 23

In bile esculin, what is used to differentiate Enterococci to other strep?

Answer 23

Esculin

Question 24

What is the positive indicator for Bile esculin?

Answer 24

Blackening of agar

Question 25

What is the reagent for Salt tolerance test?

Answer 25

6.5% NaCl broth

Question 26

What is the positive indicator for Salt tolerance test?

Answer 26

Turbidity

Question 27

What is the reagent used for Bile solubility test?

Answer 27

Na deoxycholate and Na taurocholate

Question 28

What is the positive indicator for Bile solubility test?

Answer 28

Lysis of colonies

Question 29

What is the reagent for Optochin test

Answer 29

Ehtylhydrocuprene hydrochloride

Question 30

Size and grams of Ethylhydrocuprene hydrochloride?

Answer 30

6mm, 5ug disk

Question 31

What bacteria is positive in Optochin?

Answer 31

S. pneumoniae

Question 32

What bacteria is positive in Bile solubility?

Answer 32

S. pneumoniae

Question 33

This has a positive result for Capsular swelling? Antiserum for capsule

Answer 33

Neufeld reaction (Quellung reaction)

Question 34

S. pneumoniae has capsule T or F

Answer 34

T