Part G: Lecture 22B

Question 1

single nucleotide polymorphisms (SNPs) are ___

Answer 1

bp sub between genes (they don’t affect health)

Question 2

SNPs can be used as ___

Answer 2

genetic markers

Question 3

methods for determine gene location

Answer 3

-restriction fragment length polymorphism (RFLP)
-Genome-wide association study (GWAS)

Question 4

RFLP mapping was very ____ but ____

Answer 4

useful
slow, expensive, radioactive and fussy

Question 5

GWAS purpose

Answer 5

map gene(s) responsible for a phenotype to 700K SNPs in a single step

Question 6

GWAS isolate ____ and analyze ____

Answer 6

DNA from many people
it with DNA microarray reader

Question 7

results of GWAS

Answer 7

graphs called Manhattan plots

Question 8

Manhattan plots show the ___

Answer 8

probability that the mutation is < 10 mu from each SNP

Question 9

single peak on Manhattan plot means that ____

Answer 9

one gene is involved in phenotype

Question 10

multiple peak on Manhattan plot means that ____

Answer 10

multiple genes are involved in phenotypes

Question 11

GWAS works with ____ and _____

Answer 11

model organisms
non-model organisms

Question 12

Types of maps used by geneticists

Answer 12

-cytogenetic map
-genetic map
-dna sequence map

Question 13

cytogenetic map

Answer 13

from giesma staining
ex. 15q12

Question 14

genetic map

Answer 14

close to which genetic marker
set 0 mark on chromosome
ex. 1-1.5

Question 15

DNA sequence map

Answer 15

based upon DNA sequencing
ex. Chr 18: ## bp