Pipet Classification

Question 1

Calibration Marks/Design

Answer 1

1. To Deliver
2. To Contain

Question 2

Calibration Marks/Design:

delivers the exact amount it holds a container

Answer 2

To Deliver

Question 3

Calibration Marks/Design:

holds the particular volume but does not dispense the exact volume

Answer 3

To Contain

Question 4

Calibration Marks/Design:

high standards for accuracy

Answer 4

Class A

Question 5

Drainage Characteristics

Answer 5

1. Blowout
2. Self-draining

Question 6

Drainage Characteristics

has a continuous etched rings on top of the pipet; exact volume, used for VISCOUS

Answer 6

Blowout

Question 7

Drainage Characteristics

absence of etched rings; used for NON-VISCOUS FLUID

Answer 7

Self-draining

Question 8

Types of Pipet

Answer 8

a. Measuring or Graduated Pipet
b. Transfer Pipet

Question 9

Types of Pipet

Measuring or Graduated Pipet

Answer 9

1. Serologic
2. Mohr
3. Bacteriologic
4. Ball, Kolmer, Kahn
5. Micropipet

Question 10

Types of Pipet

Transfer Pipet

Answer 10

1. Volumetric Pipet
2. Ostwald Folin
3. Pasteur Pipet
4. Automatic macropipets or micropipets

Question 11

Measure Pipet:

Graduations up to the tip
Blow-out

Answer 11

Serologic

Question 12

Measure Pipet:

Graduations doesn’t reached up to the tip
Self-draining

Answer 12

Mohr

Question 13

Measure Pipet:

May be Serologic/Mohr
volumes ranging from 1-1000 uL
To Contain

Answer 13

Micropipet

Question 14

Transfer pipet:

Bulb - middle
Non-viscous
Self-draining

Answer 14

Volumetric Pipet

Question 15

Transfer pipet:

Bulb - near tip
w/ two etched rings
Blowout

Answer 15

Oswald - Folin

Question 16

Transfer pipet:

No calibration marks ; transfer fluid without specific volume

Answer 16

Pasteur Pipets

Question 17

Transfer pipet:

Most Routinely used pipet
Automated/self-operating, semi-automatic/Manually operated

Answer 17

Automatic Macropipets/Micropipets

Question 18

Mechanisms of Automatic/Mechanical Micropipet:

Relies on piston for suction
Disadvantage: aerosol contamination

Answer 18

Air displacement pipet

Question 19

Mechanisms of Automatic/Mechanical Micropipet:

Operates moving piston or barrel like hypodermic syringe

does not require different tip for each use

Very accurate and expensive

Answer 19

Positive-Displacement Pipet

Question 20

Mechanisms of Automatic/Mechanical Micropipet:

Obtains liquid from a common reservoir

Answer 20

Dispenser/Dilutor Pipet

Question 21

Calibration of Pipets:

don’t require calibrations

Answer 21

Class A

Question 22

Calibration of Pipets:

TD pipets

Answer 22

Distilled water

Question 23

Calibration of Pipets:

TC pipets

Answer 23

Mercury

Question 24

Calibration of Pipets:

used to verify volume accuracy and precision

Answer 24

Gravimetric
Spectrometric
Photometric

Question 25

Calibration of Pipets: compare reproductibility of brands of pipet

Answer 25

0.1% Phenol Red Solution in distilled water

Question 26

Most basic pipet

Answer 26

Glass Pipet

Question 27

Pipets should be held in ____ with the tip ____ the wall of containing while draining

Answer 27

VERTICAL ; AGAINST

Question 28

It should not be shaken/hit against the wall of the container while draining

Answer 28

VOLUMETRIC TD

Question 29

Indicates pipet is not sufficiently clean

Answer 29

Imperfect wetting/Discrete droplets of water

Question 30

THERMOMETER: refrigerators and freezers

Answer 30

Total Immersion

Question 31

THERMOMETER: Heating blocks and water baths

Answer 31

Partial Immersion

Question 32

THERMOMETER: incubators and heating ovens

Answer 32

Surface Immersion

Question 33

Temperature monitoring devices should be verified for accuracy

Answer 33

6 or 12 months interval