PMLS LESSON 8 PART 4 Flashcards
Refers to Autoclaving or Steam Sterilization
wet heat
categories of thermal decontamination
wet heat and dry heat
The use of high temperatures to kill microbes
thermal decontamination
Most dependable systems available for decontamination of laboratory wastes, sterilization of laboratory glassware, media, and reagents
autoclave
What is the autoclaving setting ?
Uses saturated steam under 15 psi of pressure and 121 degrees for 30-60 minutes or depending on the material
what are the hazards possessed in Steam sterilization?
o Explosive breakage of glass vessels during opening and
unloading
o Steam burns
o Burning results from physical contact
o Metal containers, glass containers, etc.
o Aerosolization of hazardous fumes
What should containers should be used during steam sterilization and what should be the conditions?
Polypropylene bag loosely closed and glassware containing materials should be looselycapped or loosely covered in foil
sterilize items that may be damaged by moist heat or that are impenetrable to moist heat
dry heat
Relies on conduction to heat materials for periods of 2 hours at 160 degrees Celsius or 1 hour at 170 degrees Celsius
dry oven
when is dry heat used or a dry oven used?
employed when decontaminating materials that may be ruined by moist/wet heat
setup of dry oven or temp of dry oven?
160 degrees celsius for 2 hours and 170 degrees for 1 hour
Treatment of choice for animal bedding, carcasses, and
pathological wastes; but not plastics
incineration
ionizing Radiation will destroy microorganisms but is not a practical tool for laboratory use
radiation
Considered a controversial practice due to environment implications
incineration
will destroy microorganisms but is not a practical tool for laboratory use
radiation
