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Flashcards in Practical E Deck (10)
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1
Q

What are the properties of the outer/inner membrane of the mitochondria?

A

Outer: permeable to all substances. Phospolipase. Fatty acid elongation
Inner: regulation of the transport of substances

2
Q

What occurs in the intermembrane space?

A

Nucleotide metabolism. Transanimation of amino acids

3
Q

What occurs in the Inner Matrix?

A

Fatty acid oxidation. Citric cycle

4
Q

Why is the cristae of the inner membrane important?

A

Site of electron transport. Oxidation of coenzymes coupled with the reduction of water

5
Q

What instrument is used to measure the consumption of oxygen? How?

A

The oxygen elctrode. Determines the percentage saturation of oxygen in the mixture
Voltage applied through solution. Resulting current is proportional to the concentration of oxygen

6
Q

What does the Clarke elctrode comprise of ?

A

Cathode, platinum. Annonde, Silver, silver chrloide
Eletrode bathed in KCl solution
Reaction mixture separated from elctrodes by oxygen permeable, teflon membrane

7
Q

What reactions occur the annode/cathode?

A

Cathode: O2 + 4e- —-> 2 water (reduction)
Annode: 4Ag+4Cl——> 4AgCl + 4 e-

8
Q

How is the oxygen elctrode calibrated?

A

Calirated against a buffer saturated with 100% Oxygen

9
Q

What is the procedure for using a Clarke electrode?

A

Buffer and substrate is allowed to equilibrate
Mitochondrial preparation added
Consumption of O2 measured
ADP and inh, if any, added
Oxygen consumption measured till no further reaction

10
Q

What is NADH reoxidised by

A

Complex I. Reduction of complex.