practicum

Question 1

What is the dye used to counter-stain this?

Answer 1

safranin

Question 2

How would you describe this specimen?

Answer 2

gram-positive, bacillus

Question 3

What test is responsible for these results?

Answer 3

catalase test

Question 4

Which bacteria is positive for this?

Answer 4

Streptococcus pyrogenes

Question 5

Identify these.

Answer 5

shells of diatoms

Question 6

What cell is this?

Answer 6

Neutrophil

Question 7

The cell labeled “b” is a(n)

Answer 7

Neutrophil

Question 8

what type of white blood cell is located at the upper right of this image?

Answer 8

eosinophil

Question 9

What is the full Genus species of the following microbe?

Answer 9

pseudomonas aeruginosa

Question 10

name of the machine that measures light obserbance

Answer 10

spectrophotometer

Question 11

dashes = um?
10x
20x
40x
100x
4x

Answer 11

10-10um
20-5um
40-2.5um
100-1um
4-25um

Question 12

dyes used in: negative stain.

Answer 12

nigrosin

Question 13

negative stain used to

Answer 13

measure cell size

Question 14

dyes used in: endospore stain (2)

Answer 14

malachite green, safranin

Question 15

dyes used in: capsule stain
stains what color?

Answer 15

india ink - backfground, safranin capsule
red background, purple capsule,

Question 16

dyes used in: gram stain
stains what color?

Answer 16

crystal violet, iodine, alcohol, safranin
+ purple, - pink

Question 17

dyes used in: simple stain

Answer 17

methylene blue, nigrosin

Question 18

blood agar results (darkest yellow to least) Hemolysis

Answer 18

1) beta hemalysis, 2) alpha hemolysis. 3) gamma hemolysis

Question 19

MSA agar is what type of media

Answer 19

selective - 7.5% salt
differential - mannitol

Question 20

MSA agar results (3)

Answer 20

1) orange/green + fermented
2) light orange = growth
3) red = no growth

Question 21

what bacteria is positive for msa agar?

Answer 21

staphylococcus auerus

Question 22

catalase is what type of media?

Answer 22

differential

Question 23

catalase does what?
results?

Answer 23

breaks down oxiders

+ bubbles
- no bubles

Question 24

CAMP test is done on a ____ agar
what’s a positive?

Answer 24

blood
+ has an arrow

Question 25

bile esculin test 1) type of media 2) what does it do? 3) results

Answer 25

1) differential 2) hydrolyze esculin? 3) + = top black

Question 26

FTM 1) what does it do? 2) what does it include?chemcials 3) results

Answer 26

1) checks for type of aerobic/anaerobic 2) resazurin 3) + pink , - white

Question 27

EMB Plate 1) what does it do? 2) what does it include?chemcials 3) results

Answer 27

1) lactose fermentation? 2) eosin & methylyne blue 3) + purple/green. - no color

Question 28

EMB Plate results

Answer 28

+ purple/green. - no color

Question 29

EMB Plate what does it include?chemcials

Answer 29

eosin & methylyne blue

Question 30

Brewer's plate/ jar 1) what does it do? 2) what does it include?chemcials 3) results

Answer 30

1_ tests for aeronic/anaerobic 2_ thioglycotate & resazurin 3) + pink. - white

Question 31

motility agar contains

Answer 31

tetrazolium

Question 32

SIM tests for

Answer 32

sulfide production, indole, tryptophan, motility,

Question 33

SIM result: black =

Answer 33

+ sulfide

Question 34

SIM result: red after kovant reagant

Answer 34

+ indole

Question 35

MR positive result means...

Answer 35

glucose fermentation leads to mix acids

Question 36

MR results

Answer 36

+ red (Ph under5) -yellow

Question 37

VP positive result means...

Answer 37

glucose fermentation leads to acetoin

Question 38

VP results

Answer 38

+ red (gas production) -yellow

Question 39

phenol red fermentation tests for

Answer 39

fermentation of carbs

Question 40

phenol red uses a 2) results?

Answer 40

durham tube 2) + yellow - red

Question 41

1) phenylaline deaminase agar slant uses: 2) what color when positive

Answer 41

ferric chloride 10% , green +

Question 42

urea agar slant uses 2) what color when positive?

Answer 42

phenol red , pink = positive

Question 43

P&F agars are used only for

Answer 43

aerobes

Question 44

P&F agars test for

Answer 44

pyocyanin production

Question 45

P agar blue =

Answer 45

pseudomonas aeruginosa

Question 46

F agar fluorescent =

Answer 46

pseudomonas

Question 47

oxidase test .. tests for.

Answer 47

cytochrome C oxidase

Question 48

lysine Iron Agar Slant tests for /.... (3)

Answer 48

1) lysine breakdown 2) sulfide productin 3) glucose fermentation

Question 49

lysine Iron Agar Slant tests: red @ top =

Answer 49

aerobic, expresses lysine deaminase

Question 50

lysine Iron Agar Slant tests: purple @ bottom =

Answer 50

anaerobic, expresses lysine decarboxylase

Question 51

lysine Iron Agar Slant tests: yellow =

Answer 51

positive for glucose fermentatin

Question 52

nitrate broth tests for

Answer 52

denitrification / nitrogen gas production

Question 53

nitrate tests results after reagent A/B (1) ; red =

Answer 53

makes nitrate to nitride ( red)

Question 54

nitrate tests results durham tube (before zinc); air in tube =

Answer 54

nitrate to nitrogen ( air in bubble)

Question 55

nitrate tests results durham tube (after zinc); no change =

Answer 55

positive for denitrification

Question 56

What organism is this.. causes?

Answer 56

trypanosoma. ; african sleeping sickness

Question 57

Which organism is this

Answer 57

Giardia

Question 58

which organism is this?

Answer 58

trichomonas

Question 59

what is the name of the organism depicted below? it is a colonial green alga characterized by spherical colonies with daughter cells (black dots) embedded within

Answer 59

volvox

Question 60

Identify the black structure shown.

Answer 60

zygospore

Question 61

which organism is this?

Answer 61

Penicillium

Question 62

What are present in this specimen?

Answer 62

ascospores

Question 63

forms endospores through sporulation

Answer 63

bacillus megaterium

Question 64

produces prodogisin

Answer 64

serratia marcescans

Question 65

most resistant to antibiotics

Answer 65

pseudomonoas aeruguinosa