Flashcards in Protein Translation and Post-Translational Processing Deck (37)
Loading flashcards...
1
Do both subunits of ribosomes have enzymatic function? If not, which one does?
No, only large subunit does
2
What is the ternary complex?
eIF2a-GTP bound to initiator methionine-tRNA
3
What is the pre-initiation complex?
ternary complex bound to small subunit + mRNA
4
What is the initiation complex?
pre-initiation complex bound to large subunit
5
The energy needed to bind to large subunit comes from what?
hydrolysis of eIF2a-GTP
6
What site is methionine-tRNA in?
P site of large ribosomal subunit
7
How much energy is required to elongate one amino acid?
2 GTP is needed: one to bind tRNA to the A site (charged tRNA) and another to move ribosome one codon over
8
When does translation terminate?
once a stop codon has reached the A site
9
What pairs with the stop codon in termination of translation?
a protein, eRF (eukaryotic release factor)
10
Does termination of translation require energy?
yes, when the peptide is release from the P site, provided by the hydrolysis of eRF-GTP
- ribosomal subunits separate from each other
11
Which antibiotic binds to ribosomes and causes mistranslation of codons?
neomycin and gentamicin
12
Which antibiotic binds to small subunit and inhibits initiation?
streptomycin
- also causes mistranslation
13
What anitibiotic prevents peptidyl bond formation?
chloramphenicol
14
Which antibiotic blocks the A site and prevents rRNA binding?
tetracycline
15
Which toxin removes adenine bases from various positions of the rRNA in the large subunit?
ricin
16
Which toxin inactivates EF-2 by ADP-ribosylation?
Diptheria toxin
17
What are two ways to regulate translation (gene expression)?
1) prevent recognition of start codon by binding a protein to the 5' UTR of mRNA
2) phosphorylate eIF-2a (initiating factor), becomes inactive
18
Where does protein folding occur (organelle)?
cytosol and ER
19
Where does N-linked glycosylation occur?
ER, before protein folding is complete
20
In N-linked glycosylation, sugar is added to what residue in protein?
asparagine
21
Where is dolichol phosphate located (organelle)?
ER
22
Where does modification of oligosaccharide occur?
Golgi
1) high mannose type (bulky)
2) complex type
23
What disorder affects N-linked glycosylation?
congenital disorder of glycosylation (CDG)
24
Which disorder has defective trimming of oligosaccharide chain?
CDG II
25
Which disorder has defective synthesis of lipid-linked oligosaccharide precursor?
CDG I
26
Where does O-linked glycosylation occur?
Golgi
27
Which residues are sugar added to in O-linked glycosylation?
glycosyltransferases transfer sugars to serine or threonine of fully folded proteins
28
What is the first sugar of O-linked glycosylatoin?
N-acetylgalactosamine
29
What sugar does the H-antigen of the A type RBC?
GalNac
30
