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BACTERIOLOGY LECTURE > PSEUDOMONAS, VIBRIO, HAEMOPHILUS, SPIROCHETES AND MISCELLANEOUS BACTERIA > Flashcards

PSEUDOMONAS, VIBRIO, HAEMOPHILUS, SPIROCHETES AND MISCELLANEOUS BACTERIA Flashcards

1
Q

An opportunistic pathogen that infects burns, wounds, surgical incisions, and sites of catheterization.

A

Pseudomonas aeruginosa

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2
Q

It is the most common cause of infections of burns injuries and outer ear (otitis externa)

A

Pseudomonas aeruginosa

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3
Q

What is the shape of Pseudomonas aeruginosa

A

Slender, rod-shaped (bacillus)

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4
Q

What is the arrangement of cells of Pseudomonas aeruginosa

A

Singles or in pairs

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5
Q

Is Pseudomonas aeruginosa non-spore-forming or spore-forming

A

Non-spore-forming

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6
Q

The gram-staining reaction of Pseudomonas aeruginosa

A

Gram-negative

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7
Q

What is the flagella of Pseudomonas aeruginosa

A

Amphitrichous

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8
Q

The special requirements of Pseudomonas aeruginosa

A

No complex special requirements

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9
Q

What is the wide range temperature of Pseudomonas aeruginosa

A

4-40 degC

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10
Q

The optimum temperature of most strains of Pseudomonas aeruginosa

A

37 degC

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11
Q

Oxygen requirement of Pseudomonas aeruginosa

A

Obligate aerobe

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12
Q

A selective medium for Pseudomonas spp.

A

Cetrimide Agar Medium

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13
Q

Cetrimide Agar medium inhibits the growth of …

A

Gram-positive bacteria

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14
Q

The shape of Pseudomonas on Nutrient Agar Medium

A

Irregular

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15
Q

The shape of Pseudomonas on Cetrimide Agar Medium

A

Circular

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16
Q

The shape of Pseudomonas on MacConkey Agar Medium

A

Circular

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17
Q

Color of Pseudomonas of Cetrimide Agar Medium

A

Greenish blue

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18
Q

Type of hemolysis of Pseudomonas on Blood Agar Plate

A

B-hemolysis

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19
Q

What culture is used for Pseudomonas that produces dense turbidity in broth medium with a surface pellicle

A

Trypticase soy broth/ Nutrient broth

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20
Q

What culture is used for Pseudomonas that produces a characteristics of sweetish odor or a distinctive musty or earthy smell

A

Nutrient Agar

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21
Q

What culture is used for Pseudomonas that produces colorless colonies, NLF

A

MacConkey Agar

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22
Q

On Cetrimide Agar Pseudomonas produces irregular colony growth due to …

A

Swarming of the bacterium

23
Q

Enumerate the 4 pigments produced by Pseudomonas

A

Pyocyanin
Fluorescein
Pyorubicin
Pyomelanin

24
Q

The color of the pigment of Pyocyanin

A

Bluish-green

25
Q

The color of the pigment of Fluorescein

A

Greenish-Yellow

26
Q

The color of the pigment of Pyorubicin

A

Reddish-brown

27
Q

The color of the pigment of Pyomelanin

A

Brown to black

28
Q

What are the characteristics of Vibrio

A

Gram-negative
rigid
curved rods
comma-shaped

29
Q

This organism is present in marine environments and surface water with cosmopolitan distribution

A

Vibrio

30
Q

The most medically important member of genus Vibrio

A

Vibrio cholerae

31
Q

Vibrio cholerae is the causative agent of …

A

Diarrheal disease cholera

32
Q

It is characterized by sudden effortless vomiting, profuse rice-water stool, followed by rapid dehydration

A

Diarrheal disease cholera

33
Q

Two types of organisms under Vibrio cholerae

A

Vibrio cholerae (Non-O1) and Vibrio cholerae (O1)

34
Q

Strains under Biotype

A

Classical
El Tor

35
Q

Strains under Toxin

A

Toxigenic
Non-toxigenic

36
Q

Oxygen requirement of Vibrio

A

Facultative anaerobe

37
Q

The growth of Vibrio is stimulated by

A

Sodium chloride

38
Q

pH range of Vibrio

A

6.4 - 9.6

39
Q

Optimum pH range of Vibrio

A

8.2

40
Q

Temperature range of Vibrio

A

16 - 40 degC

41
Q

Optimum temperature of Vibrio

A

37 degC

42
Q

Vibrio resembles the …

A

Darting motility
Swarm of gnats

43
Q

The culture medium of Vibrio cholerae

A

Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS)

44
Q

Vibrio cholerae produces what color on (TCBS)

A

Yellow color

45
Q

Vibrio cholerae produces yellow color on (TCBS) due to

A

Sucrose fermentation

46
Q

What organism produces greenish color on TCBS

A

Vibrio parahaemolyticus

47
Q

Rapid growth occurs in about 6 hours with the formation of thick surface pellicle

A

Alkaline peptone water (pH8.5)

48
Q

Biochemical reactions of Vibrio cholerae

A

Ferments carbohydrates, producing acid with no gas
Indole +
Catalase & Oxidase +
Methyl red & Urease -

49
Q

This test can be used to identify Vibrio cholera colonies

A

String test

50
Q

Reagent of String test

A

0.5% Sodium desoxycholate

51
Q

Result of Vibrio cholerae on KIA

A

K/A

52
Q

Result of Vibrio cholerae on TSI

A

A/A

53
Q

This organism is considered as flesh-eating bacteria

A

Vibrio vulnificus

54
Q

This organism can be acquired through shark bite

A

Vibrio harveyi

BACTERIOLOGY LECTURE (4 decks)

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