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BACTERIOLOGY LECTURE > ENTERICS > Flashcards

ENTERICS Flashcards

(124 cards)

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1
Q

What are the most clinically identified Enterobacteriaceae

A

Escherichia
Klebsiella
Enterobacter
Salmonella
Shigella
Proteus
Yersinia

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2
Q

What are the characteristics of Family Enterobacteriaceae

A

Gram-negative
Short rods
Straight-sided bacilli
Non-sporulating
Facultative anaerobes
Motile
All ferment glucose
All are able to reduce nitrate to nitrite
Catalase; positive
Most are Cytochrome C oxidase; negative

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3
Q

All Enterobacteriaceae are motile except …

A

Shigella
Klebsiella
and some species of Escherichia, Salmonella, Yersinia

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4
Q

All motile species have what type of flagella

A

Peritrichous flagella

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5
Q

All species have peritrichous flagella except

A

Tatumella ptyseos

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6
Q

Tatumella ptyseos have what type of flagella

A

Monotrichous flagella

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7
Q

All Enterobacteriaceae are catalase positive except

A

Shigella dysenteriae

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8
Q

Enterobacteriaceae common antigens are

A

O: outer membrane
H: flagella
K: capsule
Vi: Capsule of Salmonella

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9
Q

What is the protein marker found on the surface of bacteria

A

Antigens

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10
Q

O antigen is also known as

A

Somatic antigen

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11
Q

It is heat stable and associated with the lipopolysaccharide or endotoxin moiety of the cell-wall

A

Somatic antigen

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12
Q

The antigen that makes organism to be virulent

A

O antigen

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13
Q

Mucus with blood defecating

A

Dysentery

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14
Q

K antigen is also known as

A

Envelop or capsular antigen

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15
Q

What kind of antigen is also known as capsular antigen

A

K antigen

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16
Q

H antigen is also known as

A

Flagellar antigen

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17
Q

What kind of antigen is also known as flagellar antigen

A

H antigen

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18
Q

It is an antigen that is heat-labile and protein in nature

A

H antigen

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19
Q

What are the indole test positive organisms

A

Klebsiella oxytoca
Citrobacter koseri
Proteus vulgaris

Escherichia coli
Morganella morganii
Providencia

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20
Q

Methyl Red positive organisms

A

Salmonella spp.
Shigella spp.
Escherichia coli
Proteus spp.
Yersinia
Citrobacter

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21
Q

Methyl Red negative organisms

A

Klebsiella pneumoniae
Enterobacter spp.
Serratia marcescens
Hafnia spp.

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22
Q

Vogues Proskauer positive organisms

A

Klebsiella pneumoniae
Enterobacter spp.
Serratia marcescens
Hafnia spp.

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23
Q

Citrate positive organisms

A

Klebsiella pneumoniae
Enterobacter spp.
Citrobacter freundii
Salmonella spp.
Serratia marcescens
Proteus mirabilis
Providencia

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24
Q

Citrate variable organisms

A

Proteus mirablis
Vibrio cholerae
Vibrio parahaemolyticus

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25
It is degradation product of the amino acid TRYPTOPHAN together with pyruvic acid and ammonia
Indole test
26
Enzyme to degrade TRYPTOPHAN
TRYPTOPHANASE
27
What broth is used in indole test
Tryptophan or peptone broth
28
What is the reagent of indole test
5 drops of Kovacs reagent containing PDAB
29
2 methods of indole test
Conventional tube method Spot indole test
30
A type of indole test method used for weak indole-producing organism
Conventional tube method
31
A type of indole test method used to detect rapid indole-producing organism
Spot indole test
32
What are the 3 most common agar for multitest agar
Sulfide-Indole-Motility (SIM) Motility-Indole-Urease (MIU) Motility-Indole-Ornithine (MIO)
33
It is the detection of end-product glucose fermentation
Methyl Red Test
34
It is a test that uses Alpha naphthol as a catalyst or color intensifier
Vogues-Proskauer
35
Acetoin is oxidized to ...
Diacetyl
36
What is the Barrits-B in Vogues Proskauer test
40% KOH or NaOH
37
Vogues Proskauer must be check/observed within ...
1 hour
38
This test determines if an organism can use sodium citrate as the sole carbon source and ammonia as the nitrogen
Citrate Utilization Test
39
What is the pH indicator of the Methyl red test
Methyl red
40
What is the pH indicator of Citrate Utilization Test
Bromthymol Blue
41
What is the alternative medium of Citrate Utilization Test
Christensen's medium
42
Christensen's medium uses what kind of pH indicator
Phenol red
43
What sugars are in TSI
Lactose Sucrose Glucose
44
This test is useful for the presumptive identification of gram-negative enteric
Triple Sugar Iron Agar
45
What is the pH indicator of TSI test
Phenol red
46
What is the H2S indicator of TSI
Ferrous sulfate and Sodium thiosulfate
47
What is the sugar ratio of TSI
10:10:1
48
A/A, Gas + organisms
Escherichia Klebsiella Enterobacter
49
K/A, H2S, Gas organisms
Salmonella Proteus Arizona Citrobacter Edwardsiella
50
K/K organism
Pseudomonas
51
Motility media have a concentration of ...
0.4% or less
52
This test is useful for the differentiation of Vibrio spp. and Aeromonas spp
Distilled water motility test
53
This test determines whether an organism can hydrolyze urea
Urease test
54
Principle of Urease test
Urea + Urease = CO2+H20+Ammonia
55
pH indicator of Urease test
Phenol Red
56
Presumptive test for Helicobacter pylori
Urease test
57
Urease test used agar
Christensen's urea agar
58
Delayed positive organism in Urease test
Klebsiella spp. Enterobacter spp.
59
Organism positive for Urease test
Proteus spp. Providencia stuartii Morganella morganii Cryptococcus spp. Corynebacterium spp. Helicobacter pylori Brucella spp.
60
This test is used to determine if a bacterium is a delayed LF or NLF
ONPG Test
61
What color is ONPG Test positive
Yellow
62
This test is used to detect the ability of organisms to utilize acetate as a carbon source
MUG Test
63
This test determines whether the organism has the ability to reduce nitrate to nitrite
Nitrate & Nitrite Reduction
64
What color is Nitrate & Nitrite Reduction positive
Red color
65
Escherichia coli was discovered by whom and when
Theodore Escherich in 1885
66
Escherichia coli was first isolated from ...
Feces of newborns
67
What is Escherichia coli's morphology on XLD and HEA
Dry yellow colonies
68
What is Escherichia coli temperature range
15-45 degC
69
Escherichia coli possesses what type of capsule
Polysialic acid capsule (K1)
70
F antigen is also known as
Fimbrial antigen
71
What form of Escherichia coli on Nutrient Agar is emulsifiable in saline
Smooth form
72
What hemolytic pattern of Escherichia coli on BAP
Beta-hemolytic
73
E.coli colonies on MAC appears ...
Flat and pink (LF)
74
Sorbitol MAC is slightly selective due to ...
Low concentration of bile salts
75
Crystal violet inhibits ...
Gram-positive organism
76
Differentiation of enteric microorganisms is achieved by the combination of ...
Sorbitol and neutral red indicator
77
What strain of E.coli does not ferment sorbitol
E.coli 0157:H7
78
E.coli 0157:H7 is associated with ...
Hemorrhagic colitis (HC) and Hemolytic Uremic Syndrome (HUS)
79
E.coli on EMB appears...
Pink with green metallic sheen
80
E.coli on Liquid Media shows ...
Homogenous turbid growth within 12-18 hours
81
After 72 hours of long incubation, what is formed in the surface
Pellicles
82
This organism is responsible for more than 80% of all community-acquired UTI
Escherichia coli
83
Escherichia coli is responsible for how many percent of community-acquired UTI
80%
84
What are the subdivided group of E.coli
Enterotoxigenic E. coli (ETEC) Enteropathogenic E.coli (EPEC) Enteroaggregative E.coli (EAEC) Enterohemorrhagic E.coli (EHEC) Enteroinvasive E.coli (EIEC) Diffusely-Adherent E.coli (DAEC)
85
What E.coli strain is known to cause "Montezuma's revenge"
ETEC
86
EPEC is known to cause ...
Nursery outbreaks diarrhea (watery/infantile diarrhea)
87
EAEC is known to ...
Produce stable and labile toxins
88
EHEC is also known as ...
Shiga toxin-producing E.coli
89
EHEC pathogenicity is caused by ...
Verotoxin production
90
EIEC invades the ...
Intestinal epithelium causing Shigella-like infection
91
The 6th class of strains of E.coli
DAEC
92
This spp. is responsible for mucoid colonies
Klebsiella
93
This spp. are all non-motile
Klebsiella
94
Klebsiella is all lactose fermenters except ...
Klebsiella rhinoscleromatis Klebsiella ozaenae
95
Most commonly isolated Klebsiella spp.
Klebsiella pneumoniae
96
Klebsiella IMVC result
Neg, Neg, Pos, Pos
97
Klebsiella is also known as ...
Friedlander's bacillus
98
Klebsiella is often found in ...
Upper Respiratory Tract
99
K. ozaenae is known to cause
Ozaena
100
K. rhinoscleromatis is known to cause ...
Rhinoscleroma
101
This spp. is routinely isolated from bloodstream and wound sites
Serratia marcescens
102
The 3rd leading cause of nosocomial infection
Serratia marcescens
103
Pigment produced by Serratia marcescens
Prodigiosin
104
This organism is often confused w/ K. pneumoniae
Enterobacter
105
This spp. is weak positive on the urease test
Enterobacter cloacae
106
A NLF, that may resemble Salmonella
Hafnia
107
Media maintenance for Hafnia
CTA (Cystine Tryptic Agar)
108
This organism has burnt gunpowder/burnt chocolate odor
Proteus
109
Proteus has swarming on BAP, but not on ....
MAC
110
Separation line of two strains of Proteus
Deine's line/phenomenon
111
This organism has acid production from mannose but does not produce gas
Morganella
112
This organism may often fund on sewage, water
Citrobacter
113
All Salmonella spp. are motile except
Salmonella pullorum Salmonella gallinarum
114
All Salmonella spp. can produce gas except
Salmonella typhi Salmonella gallinarum
115
This spp. causes salmonellosis
Salmonella typhimurium
116
This spp. causes typhoid fever
Salmonella typhi
117
Common sources of Salmonella
Poultry and Egg
118
Agar for Salmonella
Salmonella-Shigella Agar
119
A parasite of the digestive tract of humans
Shigella
120
A screening test in an immunological test
Widel test
121
The causative agent of Philippine dysentery epidemic
Group B - Shigella flexneri
122
The causative agent of the most severe type of dysentery (Japanese epidemic)
Group A - Shigella dysenteriae
123
It interferes with protein synthesis
Shiga toxin
124
Oxidase positive organisms
Pseudomonas Vibrio Neisseria Campylobacter Helicobacter

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