Quarantine/TB

Question 1

Newly imported primates must be quarantined for a minimum for how many days and where?

Answer 1

minimum of 31 days at a CDC registered primate import facility

Question 2

Once animals have completed the mandatory quarantine at the import facility, how long should they be quarantined at the receiving facility?

Answer 2

30 days or longer

Question 3

On arrival, primates should be allowed how long for acclimation?

Answer 3

72 hours

Question 4

What type of tuberculin is used for the tuberculin skin test?

Answer 4

Mammalian Old Tuberculin

Question 5

How often is the tuberculin skin test performed in quarantine

Answer 5

Every 2 weeks in alternating eyelids for the duration of the quarantine period

Question 6

How often in the tuberculin skin test evaluated and how is it evaluated

Answer 6

evaluated at 24, 48, and 72 hours and graded on a scale of 0-5 for reaction grade based on the observed changes

Question 7

What type of hypersensitivity is the TST testing for?

Answer 7

delayed type hypersensitivity

Question 8

What is a grade 0 reaction?

Answer 8

Negative; no reaction observed

Question 9

What is a grade 1 reaction?

Answer 9

Negative; bruise; extravasation of blood in eyelid from injection

Question 10

What is a grade 2 reaction?

Answer 10

Negative; varying degrees of erythema of palpebrum without swelling

Question 11

What is a grade 3 reaction?

Answer 11

Indeterminant; varying degrees of erythema of palpebrum with minimum swelling or slight swelling without erythema

Question 12

What is a grade 4 reaction?

Answer 12

Positive; obvious swelling of palpebrum with drooping of eyelid with varying degrees of erythema

Question 13

What is a grade 5 reaction?

Answer 13

Positive; swelling and/or necrosis with eyelid closed

Question 14

What happens if only one animal in a group is positive?

Answer 14

all animals in the group are considered exposed and the testing and quarantine procedures are extended.

Question 15

What are three reasons for a false negative reaction to the TST?

Answer 15

1) early or advanced disease
2) Isoniazid therapy (used to tx TB)
3) Concomitant dz such as measles or measles vaccine due to immunosuppression

Question 16

What are three reasons for a false positive reaction to the TST?

Answer 16

1) exposure to Freund’s complete adjuvant
2) trauma due to improper administration of the test
3) infection with atypical mycobacterium such as M. avium or M. kansasii
4) nonspecific reaction to the vehicle

Question 17

What is the sensitivity and specificity of the intradermal skin test?

Answer 17

sensitivity- 84%

specificity-87%