QUARTER 3 GENETIC ENGINEERING

Question 1

-Develops new varieties of organisms by using the process of selection

Answer 1

CLASSICAL BREEDING

Question 2

-Seeks to achieve expression of genetic material which is already present within a species.

Answer 2

CLASSICAL BREEDING.

Question 3

-Works primarily through insertion of genetic material.

Answer 3

GENETIC ENGINEERING.

Question 4

Employs processes that occur in nature, such as sexual and asexual reproduction.
- Controlled pollination
-Mating of Animals with desired traits

Answer 4

CLASSICAL BREEDING.

Question 5

The insertion process in genetic engineering does not occur in nature.
-Laboratory procedures

Answer 5

GENETIC ENGINEERING

Question 6

Emphasizes desirable characteristics/ gene. These characteristics are not new for the species.

Answer 6

CLASSICAL BREEDING

Question 7

Involves a direct change to an organism’s genome.

Answer 7

GENETIC ENGINEERING

Question 8

Genetic Engineering deals with the modification of g___ of an organism

Answer 8

genetic Material

Question 9

Also known as Recombinant DNA Technology

Answer 9

genetic material

Question 10

Name 2 processes involved in genetic modification processes
1. In_______ new
2. En______ present ___

Answer 10

1. Introduction of New traits
2. Enhancement of present trait
   - Increasing its expression
   - Decreasing the inhibitors of the expression.

Question 11

It is a genetic Modification processes that gives a gene

Answer 11

Desired gene

Question 12

It is a genetic Modification processes that receives a gene

Answer 12

Recipient Organism

Question 13

It is a genetic Modification processes that adds a gene

Answer 13

Adding a gene

Question 14

It is a genetic Modification processes that Deletes a gene

Answer 14

Deleting a gene

Question 15

1st Step of molecular cloning/ Recombinant dna
1. Identifying

Answer 15

1. identifying which chromosome contain the gene of interest.

Question 16

2nd Step of molecular cloning/ Recombinant dna
- Selection of

Answer 16

2. Selection of appropriate vector or vehicle which would propagate the recombinant dna

Question 17

3rd Step of molecular cloning/ Recombinant dna
3. Cutting

Answer 17

3. Cutting a cleavage DNA by restriction enzymes.

Question 18

4th Step of molecular cloning/ Recombinant dna
-Ligation

Answer 18

4. Ligation of the gene of interest with the vector.

Question 19

5th Step of molecular cloning/ Recombinant dna
-Transfer

Answer 19

5. Transfer of recombinant plasmid into the host.

Question 20

6th Step of molecular cloning/ Recombinant dna
-Selection

Answer 20

6. Selection process to screen which cell contain the gene of interest.

Question 21

Are those DNA Molecules that can carry a foreign DNA fragment when inserted into it.

Answer 21

Vectors

Question 22

are circular DNA molecules that replicate independently of the bacterial chromosome.

Answer 22

Plasmids

Question 23

Qualification of a good vector
1. Small and ______
2. Can be ____
3. Not toxic

Answer 23

1. Small and easy to isolate
2. Can be introduced into a cell
3. Not toxic to host cell

Question 24

Qualification of a good vector
4. Have one ____
5. Have one or more unique ____
6. Have a selectable marker ____

Answer 24

Qualification of a good vector
4. Have one or more origins or replication
5. Have one or more unique restriction sites.
6. Have a selectable marker
(antibiotic resistance gene)

Question 25

Methods to introduce DNA into cells 1. T 2. T 3. B / G

Answer 25

1. Transformation 2. Transfection 3. Biolistics / gene gun