regulation

Question 1

DNA methlation

Answer 1

1. cytosine fliped out of alpha
2. AA inerted into it
3. cytosine methlated and reinserted into alpha

—> makes 5 methl cytosine

Question 2

DNA methlation in Bacteria

Answer 2

1. repairs processes and identifty and repair others

2. distingush genomic DNA from invaded bacteriphage DNA

Question 3

transcrition silencing

Answer 3

epigenic silencing- methylates CpG or Cpxp6

activation: histone aceytltransferases
repression: histone deacetlases

Question 4

regulatory sequences

Answer 4

can be upstream or far away (EU)

Question 5

how is the operator recognized?

Answer 5

1. close to promotor
2. over lap promotor
3. distail to gene

Question 6

what are the two regulatory protiens?

Answer 6

helix turn helix and Zinc fingers

Question 7

helix turn helix

Answer 7

reads out DNA seq with bases in majour groove

in the n terminal and spaced out dimers

Question 8

Zinc Fingers

Answer 8

30 AA
conserved in Cys, His2 and ZIn most common in humans
-aids in oligmerizationm activate/respress trans

1. small molecules - can bind directly and con change
2. phos of others - change how they interact

Question 9

bacterial regulation two ways

Answer 9

tryppan genes and lactose metabolisum

Question 10

trytophan genes

Answer 10

Helix trun helix depnds on this
LOW: no binding ON
HIGH: binding blocks RNA pol OFF

Question 11

what does CAP do

Answer 11

enchances RNA pol when more than 100
Class I: binds upstream
ClassII: overlaps RNA pol site

can cut LacZ into glucose

Question 12

Lac operon

Answer 12

HIGH Glucose: NO cap or lactose and lac 1 binds to lac operator
LOW glucose: No lactose, has cap, lac 1 binds to lac operator