translation

Question 1

antibiotic resistance

Answer 1

1. drug inactivation and target protection

Question 2

what are the 4 stages of translation?

Answer 2

1. initation
2. elongation
3. termination
4. ribosome recycling

Question 3

ribsomes

Answer 3

carry it out and move 5-3
15AA/ sec
GTP ases help

Question 4

stage steps

Answer 4

1. initation: tRNA onto P site
2. elongation: Tu loads next tRNA into A site and catalyzes pepide bonds
3. termination: class I releases find stop codon
4. ribosome recycling: dissocate rib subints and release tRNA

Question 5

aminoacetyl tRNA

Answer 5

cloverleaf and AA attached by COOh to OH

Question 6

3 types of aminoaceyl tRNA

Answer 6

1. catalytic domain: terminal adenosine nucleotide
2. anticodon binding region:/ binds to correct tRNA
3. editing domains: cleave incorrect pairs

Question 7

initation needs?

Answer 7

methanyl tRNA, AUG, iniation factors an basomal units

Question 8

Bacteria and EU are diff bc

Answer 8

bac: tRNA met, C-A, GTPases
EU: tRNA fmet, AU

Question 9

Bacteria and EU are same

Answer 9

3 GC pairs in anticodon stem

Question 10

polycistronic

Answer 10

many ORF

Question 11

Shine dalgarno

Answer 11

purine ruch and pairs with polypyrimdine

Question 12

Bacteria Intiation:

Answer 12

TFI, TF2, TF3 displaced

mRBA by shine works with SSU

Question 13

EU Intiation:

Answer 13

elFX, mrRNA = closed loop complex
monocistronic
initation @ AUG = kozac seq sensitive
1. mRNA 5 csp, 3 poly a
2. cap= elF4E, tail= PABP
3. complex loop

pre initation comlex formed for mRNAhas elfA,B,G,E,

Question 14

protiens needed for translation gene

Answer 14

GTPase drives

1. Critical GTPases ***elF2,If2, EfTu, EF6 and p loop
2. GAPS and GEF: promote gtp hydrolysis

Question 15

elongation 3 steps

Answer 15

1. decoding- Ef-Tu loads next aminiactyl
2. peptide bond formation
3. translocation: efg2 shuffle the tRNA

Question 16

decoding

Answer 16

cognate- strong ribsomal binding and GAp promotes tRNA in A site
–> Al492, Al493, G530 confo change- codon anti codon helix

Question 17

peptide bond formation

Answer 17

peptidyl transferase- on LSU with rRNA
on the p site with active site
aplha-A attacks C carbon on P site -> new peptide

Question 18

translocation

Answer 18

mRNA bound to tTRNA in a and P move through ribsome until new stop codon and releases factors in A site

Question 19

bacteria translocation

Answer 19

2 class 1 release
RF3- GTP dissociation

Question 20

EU translocation

Answer 20

1 class 1 release
3RF3 w/ erF1
couples GTP

Question 21

ribsome recycling

Answer 21

1. ribosome + mrRNA, tRNA have ABC1
2. eRF1 bound with ATPase
3. ABCE1 dissociates