RNA processing Flashcards
(13 cards)
1
Q
benefits of RNA processing
A
diversity, regulate gene expression and acts as quailty control
2
Q
RNA splicing Bacteria
A
no introms, tRNA, rRNA
e coli encodes 3 rRNAs and tRNAs
3
Q
RNA splicing EU
A
mRNA transcribed by RNA pol II as pre mRNA
removed by: proteins, RNPs
4
Q
splicing 2 exons
A
exon 1 from intron
exon 1 reacts with exon 2
5
Q
spliceosome
A
5 snRNPs (U1-6 no 3)
- snRNPS bind with pre mRNA
- rest of it binds, BBP
- pre mRNA rearrange
- others bind to exons and 2nd transesterfication
6
Q
alternative splicing
A
more common and constiutive region must be included
7
Q
5’ cap needed for
A
- effeicnet elongation + termin
- mRNA processing
- export from nucleus
- directing translation
8
Q
5 cap add in 3 stages
A
- remove phos from 5 triphos end
- guanyl transferase attaches a GMP
- guanine T methyl transferase
9
Q
polyadenlyation sites
A
and 3’ end 200 adenosides added
- 5 Capping
- RNA processing
- 3 polyadenylation
10
Q
4 stages of RNA processing
A
- 5’ capping: elong + term
- RNA splicing - reduces transcript
- 3’ polyadenlation
- RNA editing- (after trans happens)
11
Q
RNA half life
A
- structures at 5 and 5 ends
5’: protects against exonuclease digestion
3’ end: step loops to protect 3-5 exo
3’ pol A: increase stablity in EU, decrease in Bacteria
12
Q
RNA sequence steps
A
- purify the RNA
- reverse transcribe RNA int cDNA (Rt-PCR)
- sequence DNA fragments
13
Q
qPCR
A
to amp target during pcr
at the normal end and gives mre realtive information
