Reverse-Transcriptase (RT) PCR

Question 1

What is RT-PCR?

Answer 1

When an RNA strand is reverse transcribed into its DNA complement using the reverse transcriptase (such as SuperScript).
The most sensitive technique for detecting the presence of rotavirus.

Question 2

What is transcription?

Answer 2

When DNA is used as a template to synthesize RNA.

Question 3

What is reverse transcription?

Answer 3

When a series of nucleotides is copied from an RNA template to synthesise DNA.

Question 4

What is reverse transcriptase?

Answer 4

An RNA-dependent DNA polymerase.

Question 5

What is a rotavirus?

Answer 5

Any group of RNA viruses.