Rh Blood Group System

Question 1

Most important system after ABO because of the highly immunogenic properties of the D antigen

Answer 1

Rh blood group system

Question 2

5 most commonly tested Rh antigens

Answer 2

1. D
2. C
3. E
4. c
5. e

Question 3

Gene that codes for the presence of RhD protein and D antigen

Answer 3

RHD gene

Question 4

Gene that codes for the presence of C, E, c and e

Answer 4

RHCE gene

Question 5

Individuals that inherit 1 or 2 RHD genes and 2 RHCE genes

Answer 5

D positive individuals

Question 6

Individuals that have complete deletion of RHD gene and inherit only 2 RHCE genes

Answer 6

D negative individuals

Question 7

Enumerate the wiener haplotype:
DCe

Answer 7

R1

Question 8

Enumerate the wiener haplotype:
DcE

Answer 8

R2

Question 9

Enumerate the wiener haplotype:
DCE

Answer 9

Rz

Question 10

Enumerate the wiener haplotype:
Dce

Answer 10

R0

Question 11

Enumerate the wiener haplotype:
Ce

Answer 11

r ‘

Question 12

Enumerate the wiener haplotype:
cE

Answer 12

r “

Question 13

Enumerate the wiener haplotype:
CE

Answer 13

ry

Question 14

Enumerate the wiener haplotype:
ce

Answer 14

r

Question 15

Inherited allele from each parent expressed in a single term that denotes the common Rh antigens

Answer 15

Haplotype

Question 16

Inherited haplotypes or inherited genes

Answer 16

Genotype

Question 17

Derives from cell typing results

Answer 17

Phenotype

Question 18

Antibody usually IgG1 or IgG3 but do not activate complement, usually react at AHG phase of testing, clinically significant, capable of causing HDFN and acute and delayed HTRs

Answer 18

Rh Antibodies

Question 19

Most frequent cause of HDFN prior to the advent of RhIG

Answer 19

Anti-D

Question 20

Routine monoclonal reagents used most frequently

Answer 20

Low protein reagent

Question 21

Polyspecific reagent containing IgG anti-D obtained from immunized persons

Answer 21

High protein reagent

Question 22

May be performed on red cells that are identified as D-negative

Answer 22

Weak D testing

Question 23

All pieces of the D protein are present but in reduced quantities. Persons of this type do not make anti-D.

Answer 23

Weak D

Question 24

Types of Weak D that do not produce Anti-D

Answer 24

Type 1,2,3,4.1

Question 25

Inheritance of unique RHD genes that produce some but not all epitopes of the D protein

Answer 25

Partial D

Question 26

Type of weak D that may produce anti-D but need RhIG

Answer 26

Type 4.0

Question 27

Provide the corresponding antigen of the ff gene producing antigen: RHCE*ce

Answer 27

ce or F

Question 28

Provide the corresponding antigen of the ff gene producing antigen: RHCE*Ce

Answer 28

Ce or Rhi

Question 29

Provide the corresponding antigen of the ff gene producing antigen: RHCE*cE

Answer 29

cE or Rh27

Question 30

Provide the corresponding antigen of the ff gene producing antigen: RHCE*CE

Answer 30

CE or Rh22

Question 31

Found on red cells that express the D and/or C antigen. Antibodies appear as anti-D plus anti-C but cannot be separated

Answer 31

G antigen

Question 32

Rh phenotypes which suggest that pieces of genetic material that make CEce antigens have been deleted

Answer 32

D Deletion Phenotypes

Question 33

Enumerate the D Deletion Phenotypes (1-4)

Answer 33

1. D- - 2. D • • 3. Dc - 4. Dcw -

Question 34

Fails to demonstrate the presence of any Rh antigens

Answer 34

Rhnull phenotype

Question 35

What are the 2 distinct backgrounds of the Rh null phenotype

Answer 35

1. Regulator type 2. Amorph type

Question 36

Rh-associated Glycoprotein gene is absent or mutated. RHD and RHCE genes that are present can’t produce their respective antigens

Answer 36

Rh null Regulator type

Question 37

Arises from mutations to the RHCE genes in both parents and deletion of RHD genes

Answer 37

Rh null Amorph type

Question 38

Complete the sentence: A. Rh null red cells express decreased levels of 1.___ and lack 2.___ and 3.___ antigens.

Answer 38

1. Glycophorin B 2. Fy5 3. LW (Landsteiner and Wiener)

Question 39

Complete the sentence: Persons who are Rh null experience a form of 1.___ anemia, characterized by 2.___ in peripheral blood smears

Answer 39

1. Hemolytic 2. Hereditary Stomatocytosis