RNA Processing

Question 1

Three Major Classes of RNA Modifications

Answer 1

*Single base modifications: pseudouridine
*Cleavage reactions: cutting RNA to smaller segments
*Joining reactions: covalently connecting two RNA segments together.

Question 2

What is a primary transcript?

Answer 2

RNA molecule prior to processing and maturation.

Question 3

Prokaryotic vs. Eukaryotic Transcription

Answer 3

-LOCATION: Prokaryotic transcription occurs in the cytoplasm; eukaryotic transcription occurs in the nucleus.

• RNA POLYMERASE: Prokaryotes have a single RNA Polymerase that transcribes all types of RNA. Eukaryotes have three types of RNA polymerase that transcribe different types of RNA.

-PROCESSING: Prokaryotic (bacterial) mRNA molecules are not processed prior to use. Eukaryotic mRNA molecules undergo extensive processing prior to use such as capping, splicing, and tailing.

Question 4

DRAW 5’-methylguanosine cap