RPR/Monospot

Question 1

What is RPR?

Answer 1

Rapid plasma reagin

Question 2

What is a non-treponemal test for serologic detection of the
antibody Reagin?

Answer 2

RPR

Question 3

what is produced in response to an infection of syphilis?

Answer 3

antibody Reagin

Question 4

Syphilis is caused by what?

Answer 4

Treponema pallidum

Question 5

Reagin is found in?

Answer 5

serum/plasma

Question 6

what is the vehicle or “antigen” to see results RPR
macroscopically.

Answer 6

Reagin

Question 7

is a non-treponemal screening test that requires confirmation.

Answer 7

Rapid Plasma Reagin

Question 8

If flocculation is present

Answer 8

reactive (positive)

Question 9

no flocculation is present

Answer 9

non-reactive (negative)

Question 10

What is the confirmatory test for RPR testing?

Answer 10

(FTA-ABS)

Question 11

What does (FTA-ABS) stand for?

Answer 11

Fluorescent treponemal antibody-absorbed test

Question 12

Dead T. pallidum is fixed on a slide with what?

Answer 12

patient’s serum

Question 13

fluoresce =

Answer 13

positive

Question 14

no fluoresce =

Answer 14

negative

Question 15

Which tube color is collected for serum?

Answer 15

Red

Question 16

Which tube color is collected for plasma?

Answer 16

Purple

Question 17

After centrifugation, serum should be __________,
to be an acceptable sample.

Answer 17

clear and non-hemolyzed

Question 18

FTA- ABS antigen suspension should be shaken ________,
for how many seconds?

Answer 18

vigorously
10- 15 seconds

Question 19

What are the possible results for RPR?

Answer 19

reactive
non-reactive
weak

Question 20

Flocculation is seen in?

Answer 20

RPR test

Question 21

Fluoresce is seen in?

Answer 21

FTA-ABS

Question 22

How many drops of the specimen do you place on the card?

Answer 22

1 drop

Question 23

How many drops of the antigen do you place on the card?

Answer 23

1 drop per specimen

Question 24

How long do you place the RPR test card on the rotator?
how many rpm?

Answer 24

8 minutes
100rpm

Question 25

Read the RPR reaction at the end of?

Answer 25

8 minutes

Question 26

Rotate and tilt card briefly, by hand how many times?

Answer 26

3 - 4 times

Question 27

Rotate and tilt card helps to differentiate

Answer 27

non-reactive from weakly reactive results

Question 28

How do you read the RPR Test Card?

Answer 28

MACROSCOPICALLY

Question 29

Possible results for FTA-ABS are?

Answer 29

reactive or non-reactive

Question 30

Reactive Rapid Plasma Reagin tests must be confirmed by what?

Answer 30

Fluorescent Treponemal Antibody Absorption test (FTA-ABS)

Question 31

Mechanical rotator must be calibrated to

Answer 31

100 rpm

Question 32

Antigen needle must be calibrated to

Answer 32

60 drops

Question 33

Rapid Plasma Reagin possible results:

Answer 33

reactive, weak, and non-reactive

Question 34

mononucleosis which is a self-limiting disease caused by

Answer 34

Epstein-Barr virus

Question 35

Mono is typically seen in

Answer 35

young adults and children

Question 36

Sx of Mono

Answer 36

* fever * malaise * lethargy * sore throat with exudates * enlarged lymph nodes on the neck * mild hepatitis * enlarged spleen * blotchy skin rash

Question 37

mononucleosis causes the accumulation of \_\_\_\_\_\_\_\_, causing formation of \_\_\_\_\_\_\_

Answer 37

lymphocytes and monocytes “heterophile” antibody

Question 38

Mono mix the antigen for how many secs?

Answer 38

10-15 secs

Question 39

Mono test the specimen within

Answer 39

24 hours

Question 40

blood collection for Mono must be stored between ___ to ___ Celsius?

Answer 40

2-8 degrees

Question 41

Latex reagent for Mono must be shaken how?

Answer 41

gently

Question 42

For Mono gently rotate the slide for how long? or how many rpm?

Answer 42

3 minutes 60-100rpm

Question 43

Positive result for mono

Answer 43

Shows agglutination

Question 44

Negative result for mono:

Answer 44

smooth, homogenous solution No Agglutination

Question 45

Mechanical rotator should be calibrated to how many rpm?

Answer 45

100 rpm