SAS 3: Examination of Fresh tissue and Fixation Flashcards
(80 cards)
It is the microscopic study of the normal tissue of the body
Histology
It is the microscopic study of tissue affected by disease
Histopathology
The simples, least invasive test and uses the smallest needle to simply remove cells from the area of abnormality
Fine needle aspiration
This is not always adequate to obtain a diagnosis, depending on the area being biopsied
Fine needle aspiration
remove not only cells, but also a small portion of the surrounding tissue
Core needle biopsy
This provide additional information to assist in the examination of the lesion
Core needle biopsy
takes out even more surrounding tissues. it takes some out of the abnormality, but not all
Incisional biopsy
The doctor will slice into the lesion and remove only a portion of it. if the lesion is found cancerous, further surgery may be needed to remove the lesion
Incisional biopsy
generally removes the entire area in question
Excisional biopsy
Considered the primary technique for obtaining diagnostic full-thickness skin specimen
Punch biopsy
it requires basic general surgical and suture tying skills and is easy to learn
punch biospy
the technique involves the use of a circular blade that is roated down through the epidermis and dermis, and into the subcutaneous fat, yielding a 3-4 mm cylindrical core of the tissue sample
Punch biopsy
where small fragments of tissue are shaved from a surface (usually a skin)
Shave biopsy
where tissue is scooped and spooned to remove tissue or growth from body cavity such as endometrium or cervical canal
Curettings
Fresh tissues have the advantage of being examined in the living state, thereby allowing protoplasmic activities such as
Motion
Mitosis
Phagocytosis
is a process whereby a selected tissue specimen is immersed in isotonic salt solution such as normal saline solution or Ringer’s solution in a petri dish or watch glass, carefully dissected with a needle and separated by direct or zigzag spread using an applicator stick
Teasing or dissociation
is a process whereby small pieces if tissue (not more than 1 mm in diameter) are placed in a microscopic slide and forcibly compressed with another slide or with a cover glass
Squash preparation (crushing)
this method differs depending on the nature of the material to be examined
Smear preparation
may be examined as either as fresh prep similar to that described for teased preparation, or by using a supravital staining technique
Smear
with an applicator stick or platinum loop, the material is rapidly and gently applied in a direct or zigzag line throughout the slide, attempting to obtain a relatively uniform distribution of secretion.
Streaking
A selected portion of the material is transferred into a clean slide and gently spread into moderately thick film by teasing the mucous strands with applicator stick
Spreading
This is done by placing a drop of secretion or sediment upon one slide and facing it unto another clean slide.
Pull-apart
this is a special method of smear preparation whereby the surface of a freshly cut piece of tissue is brought into contact and pressed on the surface of a clean glass slide, allowing the cells to be transferred directly to the slide for examination phase-contrast microscopy or staining for light microscopic study
Touch preparation (Impression smear)
Immediate diagnosis is accomplished
Frozen section
