SAS 5: Infiltration, Embedding, and Microtomy (Part II)

Question 1

This is after impregnating the tissue where it is placed into a mold containing the embedding medium and allowed to solidify.

Answer 1

Embedding

Question 2

Ideally, the embedding medium should match the tissue type in ____ and ____

Answer 2

strength and hardness

Question 3

Types of Blocking-Out Methods

Answer 3

1. Leuckhart’s Embedding Mold
2. Compound Embedding Ring and Base Mold
3. Disposable Embedding Molds

Question 4

It consists of two L-shaped strips of heavy brass or metal arranged on a flat metal plate and which can be moved to adjust the size of the mold to the size of the specimen

Answer 4

Leuckhart’s Embedding Mold

Question 5

Made up of a series of interlocking plates resting on a flat metal base, forming several compartments. It has the advantage of embedding more specimens at a time, thereby reducing the time needed for blocking

Answer 5

Leuckhart’s Embedding Mold

Question 6

Consists of a special stainless steel base mold fitted with a plastic embedding ring, which latter serves as the block holder during cutting

Answer 6

Plastic Embedding Ring and Base Mold

Question 7

Equipped with a warm plate to manage the impregnated specimen, and a cold plat at -5C for rapid solidification of the block.

Answer 7

Tissue Tek

Question 8

Disposable embedding molds:

Answer 8

1. Peel-Away
2. Plastic Ice Tray
3. Paper Boat

Question 9

It is a disposable thin plastic embedding molds, available in 3 different sizes, are simply peeled off one at a time, as soon as the wax has solidified, giving perfect even block without trimming. It may be places directly in the chuck or block holder of the microtome,

Answer 9

Peel-Away

Question 10

It may be recommended for busy routine laboratories. Each compartment of this may be utilized for embedding one tissue bloc, which may then be removed by bending the plastic tray once the wax has solidified or by smearing the inner mole with glycerin or liquid paraffin before embedding.

Answer 10

Plastic Ice Tray

Question 11

Normally utilized for embedding celloidin blocks but are really equally useful for paraffin wax blocks. They have the advantage of being cheap and easy to make. They provide accurate identification of specimens, thereby avoiding confusion and interchange of tissue blocks

Answer 11

Paper boat

Question 12

Process by which the processed tissue is trimmed and cut unto uniformly thin slices or “sections” to facilitate studies under the microscope

Answer 12

Microtomy

Question 13

Capable of cutting a section at a predetermined thickness by sliding the block into a cutting tool, usually a steel knife, glass and diamond blade, which is attached to the machine

Answer 13

Microtome

Question 14

Blades that are used in Microtome

Answer 14

Glass and diamond blade

Question 15

3 Essential Parts of Microtome

Answer 15

1. Block Holder
2. Knife Carrier and Knife
3. Pawl, Ratchet Feed Wheel and Adjustment screws

Question 16

Parts of microtome where the tissue is held in position

Answer 16

Block holder

Question 17

Parts of microtome for actual cutting of tissue sections

Answer 17

Knife Carrier and Knife

Question 18

Parts of microtome used to line up the tissue block in proper position with the knife, adjusting the proper thickness of the tissue for successive sections

Answer 18

Pawl, Ratchet Feed Wheel and Adjustment Screws

Question 19

5 kinds of Microtomes:

Answer 19

1. Rocking Microtome - Paldwell Trefall
2. Rotary Microtome - Minot
3. Sliding Microtome - Adams
4. Freezing Microtome - Queckett
5. Ultrathin Microtome
6. Cryostat or cold microtome

Question 20

A microtome used for cutting serial sections of large blocks of paraffin embedded tissues

Answer 20

Rocking Microtome

Question 21

Used for cutting paraffin embedded sections

Answer 21

Rotary Microtome

Question 22

Used or cutting celloidin embedded sections

Answer 22

Sliding Microtome

Question 23

Used for cutting unembedded frozen sections

Answer 23

Frozen Microtome

Question 24

Used for cutting sections for electron microscopy

Answer 24

Ultrathin Microtome

Question 25

Used for cutting sections for Electron Microscope

Answer 25

Cryostat or cold microtome

Question 26

It is the simplest microtome

Answer 26

Rocking (Cambridge Microtome)

Question 27

Most common type used for both routine and research laboratories

Answer 27

Rotary Microtome

Question 28

In rotary microtome, the thickness of section is regulated by

Answer 28

ratchet feed wheel

Question 29

used to come in contact with the ratchet wheel w/c in turn rotates the micrometer screw

Answer 29

Pawl

Question 30

make the block parallel to the microtome knife at all planes

Answer 30

Adjustment screw

Question 31

2 types of sliding microtome

Answer 31

1. Base-Sledge Microtome 2. Standard Sliding Microtome

Question 32

Mainly developed for cutting celloidin embedded tissue blocks

Answer 32

Standard Sliding Microtome

Question 33

More dangerous because of the movable knife

Answer 33

Standard Sliding Microtome

Question 34

It is the most dangerous type of microtome due to the movable exposed knife

Answer 34

Sliding Microtome

Question 35

It is used to cut undehydrated thin to semi-sections of fresh, frozen tissues, especially in instances when rapid diagnosis is required

Answer 35

Freezing Microtome

Question 36

Although other microtomes can be modified for cutting frozen section, this type will give the best results and is almost used universally

Answer 36

Freezing microtome

Question 37

A refrigerated apparatus used when for fresh tissue microtomy

Answer 37

Cryostat or Cold Microtome

Question 38

Cutting sections or sizes of cryostat

Answer 38

4 Micra

Question 39

Provides sections for fresh tissue examination esp. Fluorescent Antibody Staining techniques of Histochemical

Answer 39

Cryostat or Cold Microtome

Question 40

Capable of freezing fresh tissues within 2-3 minutes

Answer 40

Thermostat

Question 41

Used for urgent biopsies for intraoperative diagnosis

Answer 41

Cryostat or Cold Microtome

Question 42

Primarily used for cutting sections at 0.5 micra for electron microscopy

Answer 42

Ultrathin Microtome

Question 43

The specimen used in ultrathin microtome is small and fixed with ______ and embedded in plastic

Answer 43

osmium tetroxide