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P1 EXAM Flashcards

1
Q

Choose what step in tissue processing each description/instrument or reagent belongs to:

Absolute alcohol

A

Dehydration

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2
Q

Choose what step in tissue processing each description/instrument or reagent belongs to:

Chloroform

A

Clearing

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3
Q

Choose what step in tissue processing each description/instrument or reagent belongs to:

Xylene

A

Clearing

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4
Q

Choose what step in tissue processing each description/instrument or reagent belongs to:

Paraplast

A

Wax infiltration

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5
Q

Choose what step in tissue processing each description/instrument or reagent belongs to:

Paraffin wax

A

Wax infiltration

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6
Q

Choose what step in tissue processing each description/instrument or reagent belongs to:

Ester wax

A

Wax infiltration

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7
Q

Choose what step in tissue processing each description/instrument or reagent belongs to:

Toluene

A

Clearing

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8
Q

Choose what step in tissue processing each description/instrument or reagent belongs to:

Benzene

A

Clearing

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9
Q

Choose what step in tissue processing each description/instrument or reagent belongs to:

Dioxane

A

Dehydration

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10
Q

Choose what step in tissue processing each description/instrument or reagent belongs to:

Hydrochloric acid

A

Decalcification

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11
Q

The ft Are some of the difficulties encountered because of improper fixation, choose what is the most probable cause of the tf. Case: Tissue blocks are brittle and hard.

a. Prolonged fixation
b. Incomplete fixation
c. Incomplete washing
d. Wrong choice of fixation

A

a. Prolonged fixation

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12
Q

The ff are some of the difficulties encountered because of improper fixation, choose what is the most probable cause of the tf Case: Removal of substance soluble in fixing agent.

a. Prolonged fixation.
b. Incomplete fixation
c. Incomplete washing
d. Wrong choice of fixative

A

d. Wrong choice of fixative

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13
Q

The ff. Are some of the difficulties encountered because of improper fixation, choose what is the most probable cause of the ff. Case: Loss or inactivation of enzymes needed for study.

a. Prolonged fixation
b. Incomplete fixation
c. Incomplete washing
d. Wrong choice of fixative

A

d. Wrong choice of fixative

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14
Q

The ff. Are some of the difficulties encountered because of improper fixation, choose what is the most probable cause of the ff. Case: Presence of artifacts/ unwanted pigments on tissue
sections.

a. Prolonged fixation
b. Incomplete fixation
c. Incomplete washing
d. Wrong choice of fixative

A

c. Incomplete washing

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15
Q

The ff. Are some of the difficulties encountered because of improper fixation, choose what is the most probable cause of the ff. Case: Tissues are soft and feather-like in consistency.

a. Prolonged fixation
b. Incomplete fixation
c. Incomplete washing
d. Wrong choice of fixative

A

b. Incomplete fixation

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16
Q

What is the reason why bony tissue and calcium deposits need to be decalcified before proceeding to tissue processing?

a. To prevent microtome knives from getting blunt.
b. To prevent bone dust from obscuring important structures.
c. To facilitate normal cutting of tissues.
d. All of the above

A

d. All of the above

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17
Q

It is considered the most common and fastest decalcifying agent.

a. Nitric acid
b. Calcium acetate
c. Hydrochloric acid
d. Chromic acid

A

a. Nitric acid

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18
Q

Perenyi’s fluid is an example of?

a. Fixative
b. Decalcifying agent
c. Clearing agent
d. Dehydrating agent

A

b. Decalcifying agent

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19
Q

Von Ebner’s fluid is composed of NaCl and Hcl this fluid is an example of a:

a. Fixative
b. Decalcifying agent
c. Clearing agent
d. Dehydrating agent

A

b. Decalcifying agent

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20
Q

EDTA disodium salt can be used as an effective:

a. Fixative
b. Decalcifying agent
c. Clearing agent
d. Dehydrating agent

A

b. Decalcifying agent

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21
Q

Dioxane can be used as a:

a. Fixative
b. Decalcifying agent
c. Clearing agent
d. Dehydrating agent

A

d. Dehydrating agent

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22
Q

Benzene can be used as a:

a. Fixative
b. Decalcifying agent
c. Clearing agent
d. Dehydrating agent

A

c. Clearing agent

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23
Q

Xylene can be used as a :

a. Fixative
b. Decalcifying agent
c. Clearing agent
d. Dehydrating agent

A

c. Clearing agent

24
Q

Bouin’s fluid is used as a:

a. Fixative
b. Decalcifying agent
c. Clearing agent
d. Dehydrating agent

A

a. Fixative

25
Heidanhans susa solution is used as a: a. Fixative b. Decalcifying agent c. Clearing agent d. Dehydrating agent
a. Fixative
26
What is the general purpose of infiltrating tissues with paraffin wax? a. To fill cavities and make tissues sturdy b. To facilitate better staining c. To improve tissue texture d. To make tissue heat resistant
a. To fill cavities and make tissues sturdy
27
Impregnation or wax infiltration is done using? a. Melted paraffin wax b. Paraffin wax pellets c. Solid wax d. Wax cooled at room temperature.
a. Melted paraffin wax
28
What is the most appropriate type of wax to help infiltrate hard tissues? a. High melting point waxes b. Low melting point waxes
a. High melting point waxes
29
Tissue+Wax a. Wax infiltration b. Embedding
a. Wax infiltration
30
Tissue with wax+ wax a. Wax infiltration b. Embedding
b. Embedding
31
All of the following can be used as a substitute for paraffin wax, except? a. Ester wax b. Paraplast c. Embeddol d. All of the above e. none of the above
e. none of the above
32
Paraffin embedded tissues are arranged _________ pf the mold together with their proper labels and immersed in melted paraffin a. Center b. Half way c. Bottom d. Top part
c. Bottom
33
Consist of two L. Shaped strips of heavy brass pr metal arrange on a flat metal plate, used mainly for embedding. a. Peel away molds b. Leukuharts mold c. Ice tray d. Paper boat
b. Leukuharts mold
34
Impregnation/ inflation technique recommended for bones, teeth, large brain sections. a. Wet celloidin method b. Dry celloidin method c. Paraffin infiltration d. Ester wax
a. Wet celloidin method
35
All of the following, refers to fixation: except: a. Prevents breakdown of cellular elements b. Coagulate or precipitate protoplasmic substances c. Preserve the tissue d. None of the above
d. None of the above
36
Routine fixation usually happens at room temperature; which of the following ranges below is closest to a room, temperature. A. 0-4C B. 30-35C C. 20-25C D. 36.5-37
C. 20-25C
37
Hypertonic solution make cells swell, while hypotonic solution make the cell shrink a. Both statements are correct b. Both statements are incorrect c. Only the first statement is correct d. Only the second statement is correc
b. Both statements are incorrect
38
Duration of fixation can be prolonged by: a. Heat b. Vacuum c. Agitation d. All of the above e. None of these
e. none of this
39
The volume of the tissue and fixative ratio is: a. 20:1 b. 1:20 c. 1:1 d. Depends on the condition
b. 1:20
40
Which of the following statements below are/is true? a. Brain must be fixed before frossing and sectioning b. Bone marrow continues to undergo mitosis up to 30 mins after death after refrigeration, c. Fixation can be retarded by the presence of mucus, d. All of the above statements are correct, e. None of the statements are correct
d. All of the above statements are correct,
41
Which of the following are the composition of 10% Formalin used commonly in laboratories? I. Formaldehyde 40%. Ill. Distilled water. II. Glutaraldehyde3% IV. Tap water a. land Il b. l and Ill c. all of the above
b. l and Ill
42
Which of the following are the composition of 10% Buffered Formalin used commonly in laboratories? I. Formaldehyde 40% II. Na dihydrogen phosphate III. Distilled water IV. Sodium hydrogen phosphate V. Disodium hydrogen phosphate I and Il a. I and II b. I,II AND Ill c. all of the above d. All except IV
d. All except IV
43
What is the advantage of using buffered formalin compared to the normal formalin. A. Buffered formalin is much faster in fixation time. B. Buffered formalin is less toxic than normal formalin. C. Buffered formalin does not form pigments. D. Buffered formalin forms pigments
C. Buffered formalin does not form pigments.
44
It is the process of removing water from intracellular and extracellular compartments of the tissue. A. Dehydration B. Fixation C. Decalcification D. Impregnation
A. Dehydration
45
Usually appears in the head and neck and may persist for 3-4 days
Livor mortis
46
Correctly pair the following to its most common functions/use on. NaCI
Formol-Saline
47
Correctly pair the following to its most common functions/use on. NaCI
Formol-Saline
48
Correctly pair the following to its most common functions/use on. Calcium acetate
Formol-calcium
49
Correctly pair the following to its most common functions/use on. Mercuric chloride + Na acetate
B-5 fixative
50
Correctly pair the following to its most common functions/use on. Picric acid in 95% Alcohol
Gendre's fluid
51
Correctly pair the following to its most common functions/use on. Saturated Mercuric chloride
Formol-sublimate
52
Correctly pair the following to its most common functions/use on. CNS
Formol-Saline
53
Correctly pair the following to its most common functions/use on. Red Blood cells
Formol-sublimate
54
Correctly pair the following to its most common functions/use on. Lipid fixation
Formol-calcium
55
Correctly pair the following to its most common functions/use on. Glycogen
Gendre's fluid
56
Correctly pair the following to its most common functions/use on. Bone Marrow
B-5 fixative

Histopathology (22 decks)

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