Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

HISTOPATH > SECTIONING > Flashcards

SECTIONING Flashcards

1
Q

embedded tissue is trimmed and cut into uniformly thin slices using a microtome

A

sectioning

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

3 essential parts of microtome

A
  • block holder (aka chuck)
  • knife carrier and knife
  • pawl, ratchet feed wheel and adjustment screws
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

inventor of rocking microtome

A

paldwell trefall

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

simplest and most popular type

A

rocking microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

routinely used microtome

A

rotary microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

inventor of rotary microtome

A

Minot

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

for celloidin sections and hard rough tissue blocks

A

sliding microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

inventor of sliding microtome

A

Adams

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

two types of sliding microtome

A

base sledge/sledge type and standard sliding

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

for frozen sections; CO2 is used as a propellant

A

Freezing Microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

invented by Quackelt

A

freezing microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

what is inside of a cryostat/cold microtome

A

rotary microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

microtome primarily for electron microscopy

A

ultrathin microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

special knife used in ultrathin microtome

A

diamond knife

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

for enzyme histochem

A

vibratome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

very hard material (undecalcified bone); tissue embedded in resins

A

saw microtome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

25 mm

A

plane concave

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

120 mm

A

biconcave knife

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

knife used for rotary microtome

A

biconcave knife

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

used for rocking microtomes with a fixed handle

A

Heiffor Knife

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

100 mm

A

plane wedge

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

for frozen sections or very hard tissues

A

plane wedge

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

knives for ultrathin microtome

A

glass and diamond knives

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

found on the tapered edge of a knife

25
angle formed between the cutting edge
bevel angle
26
optimum angle for cutting angle
15 degrees
27
angle formed between the surface of the block and the cutting edge of the knife; 0.15 degrees
clearance angle
28
angle of the upper surface of the facet and surface of tissue block
rake angle
29
angle between the sides of knife
wedge angle
30
process of removing nicks (irregularities of knife)
Honing
31
movement of Honing
from HEEL TO TOE
32
number of strokes per surface
10-20 strokes
33
hones for best result
belgium yellow
34
hones with polishing effects
arkansas
35
hones for badly nicked
fine carborundum
36
used for removal of burrs (irregularities of knife formed after honing)
stropping
37
movement from top to heel
stropping
38
paddle strop is made of
hoarse leather
39
rapid diagnosis; for enzyme histochemistry
frozen section
40
point in which sections may be cut at 10 um; frozen tissue starts to thaw (warmed with the finger) and becomes invisible to the naked eye
dew line
41
used to remove the ribbon that sticks to the knife blade
camel hairbrush
42
optimum temp of cryostat
-18 to -20C (20C)
43
most rapid method of freezing
liquid nitrogen
44
not for muscle; made of fluorinated hydrocarbons
aerosol sprays
45
methods of freezing
- liquid nitrogen - isopentane - aerosol sprays - carbon dioxide - freon
46
clearing agents used in frozen section
glycerin and gum syrup (water soluble)
47
use of synthetic water soluble glycols and resins
mounting
48
recommended mounting medium
OCT (optimal cutting temperature compound)
49
difficulties due to faulty fixation, dehydration, clearing, embedding
- brittle; hard tissue - clearing agent becomes milky - soft and mushy tissue - tissue becomes opaque - very hard tissues; tissue shrinkage - air holes on trimmed tissue block - moist block that tends to crumble tissue block smells like xylene
50
horizontal or parallel lines across the sections
chatters
51
most common tissue adhesive
mayer's egg albumin
52
components of mayer's egg albumin
- egg white - glycerol - thymol crystals
53
added to increase viscosity and prevent complete drying
glycerol
54
used as a general purpose section adhesive
poly-L-lysine
55
widely used as an adhesive in immunohistochem
poly-L-lysine
56
stops the chemical reaction and diffusion in tissue
quenching
57
most time consuming; introduction of heat to cause sublimation of heat; removal of water vapor from surface of specimen
drying
58
freezing substitution makes use of ___________
rossman's formula or in 1% acetone and dehydrated in absolute alcohol

HISTOPATH (17 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions