Semen collection and eval

Question 1

What is semen and what is it composed of?

Answer 1

composed of spermatozoa that comes from the testes and seminal plasma that comes from the accessory sex glands

Question 2

Most commonly __ is used for sperm collection in Bulls and the sperm rich fraction will be ___

Answer 2

EEJ, cloudy/white

Question 3

Semen collection in a ram can be __ or __, you may need __

Answer 3

AV, Electroejaculator, sedation

Question 4

semen collection in boar is often done by a __ or ___ under anesthesia

Answer 4

gloved hand, electroejaculator

Question 5

stallion semen collection is often accomplished by __

Answer 5

training the male to mount a phantom or female in estrus

Question 6

dog semen collection is often done by __ or a __ and the sperm you’re most interested will look __

Answer 6

manual stimulation, teaser, milky

Question 7

what 4 things do we look at on semen evaluation?

Answer 7

1. gross appearance
2. volume/sperm concentration (AV)
3. sperm motility
4. morphology

Question 8

bull semen can slightly vary in color (looking yellow) because of __ or it may be contaminated with urine

Answer 8

riboflavin

Question 9

mass motility class depends on __ and __

Answer 9

individual sperm motility and concentration of sample

Question 10

to assess sperm viability use a __ stain. __ sperm exclude the stain and look white while __ sperm take up the stain and are the same color as the background

Answer 10

vital, live, dead

Question 11

sperm concentration

Answer 11

calculation of sperm per mililiter of ejaculate

Question 12

sperm morphology is the __ thing to look at. Make sure you u use the __ magnification

Answer 12

most important, highest

Question 13

primary abnormalities come from __ in the testis and result in __, midpiece and __ defects

Answer 13

spermatogenesis, head, severe tail

Question 14

secondary abnormalities arise within the epididymis during __ resulting in __, __ and __

Answer 14

storage/maturation, detached heads, distal droplet, and mild tail defects

Question 15

compensable sperm morphology is typically not __ while non-compensable has non-viable sperm __ with normal sperm

Answer 15

competing

Question 16

passing spermiogram reqs for stallions

Answer 16

> 60% progressive motile concentration should match predicted DSO

~ 50% morphologically normal

Question 17

bulls/rams/bucks passing spermiogram requirements

Answer 17

> 70% morphologically normal sperm
30% progressively motile

Question 18

oligiospermia

Answer 18

reduced numbers of sperm in ejaculate with high ALP

Question 19

complete infertility is __

Answer 19

uncommon