Serology Flashcards
(15 cards)
Serology Direct Tests
target antigens
Serology Indirect Tests
target antibodies
Acute Infection
Antigen; pathogen; 4 fold increase between acute and convalescent serum titers
Direct Fluorescent Dyes
see and identify antigen directly
Indirect Fluorescent Dyes
see and identify attached antibody
ELISA
immunoassay
detect Ag or Ab
Direct ELISA
probes for Ag with 1 labeled Ab
Indirect ELISA
probes for Ab
labeled Ab binds to unlabeld Ag
Sandwich ELISA
Ag sandwiched between 2 Ab
most common
Agglutination
Particulate Ag (RBCs) + Ab soluble antigens + bound Ab
Prozone
high levels of Ab saturate Ag to point where Ag do not show up on tests
Equivalence
no free Ag or Ab remain in solution
max precipitation
Lattice Theory
Should have max crosslink- same # Ag and Ab
Precipitin Curve
serum is serially diluted with saline and a fixed amount of Ag added to each tube
tests agglutination
High Antibody Titer means…..
Agglutination test is negative
