Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

BACTERIOLOGY > Tests for Micrococcus and Staphyloc > Flashcards

Tests for Micrococcus and Staphyloc Flashcards

1
Q

Test for Micrococcus and Staphylococcus

A

Catalase Test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Screening test for Gram-positive organisms, differentiates Micrococcaceae from Streptococci

A

Catalase Test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Chemical reaction in Catalase Test

A

O2 + H2O2 → H2O and O2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Reagent for Catalase Test

A

3% or 30% hydrogen peroxide, 22-25°C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Positive QC for Catalase test: Copious bubbles/effervescence (positive)

A

S. aureus; False pos loop contaminated with blood agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Negative QC for Catalase test No or weak effervescence (negative)

A

Streptococci (e.g., S. pyogenes)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Differentiate Micrococci from Staphylococci

A

Microdase (Modified Oxidase) test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Chemical reaction in Microdase test

A

Oxidase + reagent + Cytochrome C → Indophenol blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Incubation conditions for Microdase test

A

22-25°C, 2 minutes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Positive QC for Microdase test: Blue to Purple-blue (positive)

A

Micrococcus luteus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Negative QC for Microdase test: No color change (negative)

A

S. aureus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Differentiate S. aureus from CoNS

A

Coagulase test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Chemical reaction in Coagulase test

A

Coagulase activates coagulase-reacting factor (CRF), a modified thrombin, which activates fibrinogen to fibrin clot

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Reagent for Slide method coagulase test

A

Drop of EDTA Rabbit plasma

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Incubation conditions for Slide method coagulase test

A

22-25°C, 5-10 seconds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Positive QC for Slide method: Clumping (positive)

A

S. aureus in rabbit plasma

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Negative QC for Slide method: No clumps (negative)

A

S. epidermidis in water/saline

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Reagent for Tube method coagulase test

A

0.5 mL EDTA Rabbit plasma

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Incubation conditions for Tube method coagulase test

A

35-37°C, 1-4 hours

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Positive QC for Coagulase Tube method: Clot (positive)

A

S. aureus in rabbit plasma

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Negative QC for Coagulase Tube method: No clot (negative)

A

S. epidermidis in water/saline

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Distinguish S. aureus, Serratia, and M. catarrhalis

A

DNase test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Chemical reaction in DNase test

A

DNA/Methyl Green complex + deoxyribonuclease → colorless

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Media for DNase test

A

DNase agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Reagent for DNase test
Methyl green
26
Incubation conditions for DNase test
35-37°C, 13-24 hours
27
Positive QC for DNase test/Methyl green: Colorless or Clearing (positive)
S. aureus
28
Negative QC for DNase test/Methyl green: : Green (negative)
E. coli
29
Chemical reaction in DNA/Toluidine Blue test
DNA/Toluidine Blue complex + deoxyribonuclease → colorless
30
Method for DNA/Toluidine Blue test
Agarose slant
31
Reagent for DNA/Toluidine Blue test
Toluidine blue
32
Positive QC for DNA/Toluidine Blue test: Deep pink (positive)
S. aureus
33
Negative QC for DNA/Toluidine Blue test: Blue (negative)
E. coli
34
Result for DNAse Hydrochloric acid test
No precipitate of DNA
35
Aids in ID of some GP cocci and bacilli
Voges-Proskauer (Coblentz method)
36
Chemical reaction in Voges-Proskauer test
Carbohydrate → 2,3-Butanediol or acetoin (end product)
37
Reagent for Voges-Proskauer test
MRVP medium, Solution A: α-naphthol, Solution B: 40% KOH with creatine
38
Incubation conditions for Voges-Proskauer test
22-25°C, 30 minutes
39
Positive QC for Voges-Proskauer test: RED (positive)
B. anthracis, B. cereus, Staphylococcus aureus, L. monocytogenes, Streptococcus anginosus
40
Negative QC for Voges-Proskauer test: Yellow (negative)
E. coli
41
Differentiates Clumping factor positive Staphylococci
PYR test
42
Chemical reaction in PYR test
L-Pyrrolidonyl-β-naphthylamide + L-pyrrolidonyl arylamidase → β-naphthylamine (detected by indicator)
43
Media for PYR test
Filter paper PYR disk
44
Reagent for PYR test
N,N-methylamino-Cinnamaldehyde
45
Incubation conditions for PYR test
22-25°C, 2 minutes
46
Positive QC for PYR test: Bright Red (positive)
S. intermedius, S. lugdunensis
47
Negative QC for PYR test: No color change (negative)
S. aureus
48
Coagulase test AKA
clumping factor, bound coagulase, slide method
49
Location of bound coagulase
Found on the surface of the cell, detected in slide coagulase
50
Slide Coagulase Procedure
Bacteria + drop of EDTA rabbit plasma on a glass slide or via latex agglutination
51
Slide Coagulase Incubation
Room temperature, immediate reaction
52
Positive result for Slide Coagulase
Agglutination (fibrin clumps)
53
Positive organism, PYR negative (Slide Coagulase)
S. aureus
54
Positive organism, PYR positive (Slide Coagulase)
S. lugdunensis, S. schleiferi, S. intermedius
55
Tube Coagulase test
Detects free coagulase, also known as staphylocoagulase or extracellular coagulase
56
Tube Coagulase Procedure
Bacteria + 0.5 mL EDTA rabbit plasma in a tube
57
Tube Coagulase Incubation
35°C for 1-4 hours (false negatives possible if >4 hours)
58
Positive result for Tube Coagulase
Clot formation
59
Positive organism (Tube Coagulase)
S. aureus (VP positive)
60
Negative organism (Tube Coagulase)
S. intermedius
61
Confirmation protocol for negative Coagulase test
Negative slide coagulase → tube coagulase → overnight incubation at room temperature → still no clot = coagulase negative
62
Media for Corynebacterium
Loeffler serum slant
63
Colony color of S. aureus
Golden yellow
64
Colony color of S. citreus
Lemon yellow
65
Colony color of S. albus (S. epidermidis)
Porcelain white

BACTERIOLOGY (49 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions