The ECM

Question 1

contacts basal lamina

Answer 1

basal surface

Question 2

basil lamina physical properties

Answer 2

very thin, tough flexible sheet

Question 3

these rest on basal lamina foundation

Answer 3

columnar epithelia

Question 4

two distinct basal lamiae form a tight barrier that limits:

Answer 4

diffusion (between blood and brain)

Question 5

role basal lamina plays in tissue repair after wounding

Answer 5

cell adhesion

Question 6

basal lamina cell adhesion after:

Answer 6

wounding

Question 7

basal lamina functionally provide a surface for:

Answer 7

fibroblast migration

Question 8

basal lamina composed of 4 major proteins:

Answer 8

Type IV collagen, Laminins, Perlecan, Nidogen

Question 9

trimeric molecules with rodlike and globular domains that form a 2D network

Answer 9

Type IV collagen

Question 10

family of multiadhesive cross-shaped proteins that form fibrous 2D network with Type IV collagen

Answer 10

Laminins

Question 11

large multidomain proteoglycan that binds to and cross-links many ECM components

Answer 11

Perlecan

Question 12

structure of collagen proteins

Answer 12

trimeric proteins

Question 13

three collagen polypeptides form into:

Answer 13

triple helix

Question 14

repeating sequence of collagen peptide

Answer 14

Gly - X - Y

Question 15

collagen peptide sequence X and Y stand for:

Answer 15

proline and hydroxyproline (or lysine and hydroxylisine)

Question 16

collagen peptide amino acid small enough to fit in center of triple helix

Answer 16

Gly

Question 17

collagen triple helix monomer associate in this manner

Answer 17

head-to-head or tail-to-tail

Question 18

collagen small globular domain at N-terminus forms:

Answer 18

tetramers

Question 19

large globular domain at C-terminus forms:

Answer 19

dimers

Question 20

collagen dimers and tetramers associate to form:

Answer 20

lattice

Question 21

some collagen covalent crosslinking occurs between these domains

Answer 21

C-terminal globular domains

Question 22

collogen mutations in Gly cause: (scientific and common name)

Answer 22

osteogenesis imperfecta (brittle bone disease)

Question 23

mutation in Gly from brittle bone disease can disrupt:

Answer 23

collagen triple helix

Question 24

brittle bone disease S/S

Answer 24

bones break from normal weight-bearing

Question 25

deficiency in co-factor leads to scurvy

Answer 25

Vitamin C (ascorbic acid)

Question 26

enzyme type requires Vitamin-C

Answer 26

hydroxylase

Question 27

scurvy causes these to not be hydroxylated

Answer 27

prochains

Question 28

Scurvy: these do not form stable structures

Answer 28

collagen triple helices

Question 29

Scurvy: these physical body structures become fragile

Answer 29

blood vessels, tendons, and skin

Question 30

S/S of Alport Syndrome

Answer 30

progressive loss of kidney function, hearing loss, and blindness

Question 31

Alport Syndrome caused by mutations in these genes

Answer 31

COL4A5 (X-linked) and COL4A3/4 (autosomal)

Question 32

S/S of Collagen II mutants

Answer 32

shortened/deformed bones

Question 33

bones severly affected when embryos null of this

Answer 33

Perlecan

Question 34

fibronectan is present on:

Answer 34

fibroblasts

Question 35

normal fibroplasts adhere tightly because of:

Answer 35

fibronectan

Question 36

fibronectin is absent from surfaces of:

Answer 36

cancerous fibroblasts

Question 37

FN essential for these processes:

Answer 37

cell migration, differentiation, wound healing, (and clotting)

Question 38

fibronectin-cultured salivary glands undergo:

Answer 38

branching morphogenesis

Question 39

salivary gland culture with anti-Fibronectan Ab blocks:

Answer 39

branching

Question 40

structure of Fibronectin

Answer 40

dimer

Question 41

Fibronectan dimer polypeptide chains linked by:

Answer 41

disulfide bond (at C-terminus)

Question 42

Fibronectin connects to cytoskeleton via:

Answer 42

focal adhesions

Question 43

Focal adhesions are:

Answer 43

points of contact between cell and surface (ECM or tissue culture plate)

Question 44

form of fibronectin circulates in blood and functions in clotting

Answer 44

soluble form

Question 45

insoluble fibronectin assembles into fibrils only on:

Answer 45

surface of cells that can find to it

Question 46

Type III fibronectin repeats contain this sequence

Answer 46

Arg-Gly-Asp (RGD)

Question 47

Type III fibronectin repeats important for:

Answer 47

cell binding

Question 48

this caused when actin cytoskeleton transmits tension to fibronectin (stretching it)

Answer 48

binding sites exposed

Question 49

this forms after fibronectin binding sites exposed

Answer 49

fibronectin filament

Question 50

experiment proves RGD sufficient to mediate adhesion: different concentrations of:

Answer 50

synthetic peptides with various sequences

Question 51

experiment proves RGD sufficient to mediate adhesion: synthetic peptides crosslinked to:

Answer 51

antibody attached to dish surface

Question 52

to move through tissue cell has to grab and let go of:

Answer 52

ECM

Question 53

integrin outside-in activation controled by:

Answer 53

RGD peptide in extracellular domain

Question 54

integrin inside-out activation controled by:

Answer 54

regulatory molecules (like PIP2)

Question 55

integrin outside-in activation: in this type of confiuration intracellular domain of two subunits separate and expose binding site for talin on beta-subunit

Answer 55

unfolded, active configuration

Question 56

integrin outside-in activation: binding of talin triggers assembly of:

Answer 56

actin filaments anchored to integrin

Question 57

integrin outside-in activation: this exerts tension when extracellular domain binds ligand

Answer 57

cytoskeleton

Question 58

integrin inside-out activation: this can compete with alpha subunit

Answer 58

talin

Question 59

integrin inside-out activation: talin competes with alpha subunit for

Answer 59

beta subunit

Question 60

integrin inside-out activation: if this happens, integrin configuration forced to open

Answer 60

talin binds to beta subunit

Question 61

integrin inside-out activation: regulatory molecules (like PIP2) activates talin by:

Answer 61

increasing affinity for beta-subunit

Question 62

overall structure of insoluble fibronectin binding complex at cell membrane

Answer 62