The Microscope/ Staining

Question 1

How do you calculate the total magnification?

Answer 1

Total magnification is achieved by the multiplication of the power of the eyepiece lens by the power of the objective lens.

Example- 10x4= x40

Question 2

What is an electron microscope and what is the advantages and disadvantages?

Answer 2

The electron microscope uses a beam of electrons Instead of light. An advantage is It is possible to get much larger magnifications. The disadvantages of an electron microscopes is that you could only study dead cells.

Question 3

Iodine solution

Answer 3

It makes the nucleus more obvious and stains and starch present mostly used to stain plant cells

Question 4

Methylene blue

Answer 4

Stains acidic parts of the cell blue makes the nucleus more obvious, mostly used to prepare slides of animal cells and bacteria.

Question 5

How do I calculate magnification of a drawing or picture?

Answer 5

Magnification= length of A-B on picture
Divided by
Real length of A-B

Question 6

Why are electron microscopes useful

Answer 6

Electron microscopes letters, study cells and much finer detail than light, microscopes, enabling biologist to see and understand more sub cellular structures, such as ribosomes and plasmids

Question 7

What is the purpose of the fine adjustment knob on a light microscope

Answer 7

It’s used to focus the lens until a clear image of what’s on the slide appears

Question 8

True or false electron microscopes have a smaller magnification than light microscopes, but they have a higher resolution than light microscopes

Answer 8

False electron microscopes have both a higher resolution and a higher magnification than a light microscope