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Flashcards in Transcription Deck (37):
1

what does RNA contain instead of a deoxyribose

ribose

2

what is the structure of RNA

single stranged

3

what is transcription catalyzed by

RNA polyermase

4

what does RNA polyermase do

moves along templant DNA and unwinds it. Also catalyzes formation of phosphdiester bonds increasing the length of RNA polyer

5

difference between RNA polymerase and DNA polymerase

RNA polymerases initate RNA synthesis without a primer. more error prone

6

RNA polyermase used in mRNAs

POl II

7

RNA polyermase unsed in rRNAs

Pol I mainly, some Pol III

8

RNA polyermase used in tRNAs

pol III

9

RNA polyermase used in snoRNAs

pol II

10

RNA polyermase used in other non-coding RNAs

pol III and II

11

function of rRNA

components fo ribosome that are required for protein synthesis

12

function of tRNA

serve as adapters between mRNA and amino acids during protein synthesis

13

function of snRNAs

serve a number o ffunction in the nucleus, including pre-mRNA splicing

14

function of snoRNAs

process and modify rRNAs

15

what is the most abundent RNA in the cell

rRNA

16

how is tRNA molecules synthesized

larger precurosor molecules are trimmed and chemically modified

17

how does pol II know where to start

promoter region of DNA

18

what is common in promoter regions

consensus DNA

19

consensus sequence

sequence is similiar but not necessarily identifcal

20

examples of consensus sequres

TATA box, CAAT box, GC region

21

what consensus sequence is alomst always found in genes transcribed by Pol II

TATA box

22

does pol II requre transcription factors in order to initiate transcription

yes

23

function of TBP portion of TFIID

binds to TATA box, acts as a signpost

24

what base pairs are easier to pull apart

A-T

25

what are promotor regions usually rich in

AT pairs

26

basal transcription complex

compex of RNA and general transcription factors

27

function of basal transcription complex

initiates transcription at low rate

28

what do GC-rich sequences bind

SP-1

29

what does CAAT box bind

NF1

30

function of enchanger elements

proteins bind here and stimulate transcription a great deal

31

what is a prerequisite to transcription

extensive chromatin remodeling

32

toxin found in death cap mushroom

alpha-amanitin

33

how does alpha-amnitin work

inhbitor of Pol II

34

what happens when you inhibit pol II

block synthesis of mRNA

35

how does rigampicin work

potent inhibitor of RNA polyermase

36

does rifampicin affect eukaryotic pol II

no

37

what do you use rifampicin for

TB