Transcription

Question 1

what does RNA contain instead of a deoxyribose

Answer 1

ribose

Question 2

what is the structure of RNA

Answer 2

single stranged

Question 3

what is transcription catalyzed by

Answer 3

RNA polyermase

Question 4

what does RNA polyermase do

Answer 4

moves along templant DNA and unwinds it. Also catalyzes formation of phosphdiester bonds increasing the length of RNA polyer

Question 5

difference between RNA polymerase and DNA polymerase

Answer 5

RNA polymerases initate RNA synthesis without a primer. more error prone

Question 6

RNA polyermase used in mRNAs

Answer 6

POl II

Question 7

RNA polyermase unsed in rRNAs

Answer 7

Pol I mainly, some Pol III

Question 8

RNA polyermase used in tRNAs

Answer 8

pol III

Question 9

RNA polyermase used in snoRNAs

Answer 9

pol II

Question 10

RNA polyermase used in other non-coding RNAs

Answer 10

pol III and II

Question 11

function of rRNA

Answer 11

components fo ribosome that are required for protein synthesis

Question 12

function of tRNA

Answer 12

serve as adapters between mRNA and amino acids during protein synthesis

Question 13

function of snRNAs

Answer 13

serve a number o ffunction in the nucleus, including pre-mRNA splicing

Question 14

function of snoRNAs

Answer 14

process and modify rRNAs

Question 15

what is the most abundent RNA in the cell

Answer 15

rRNA

Question 16

how is tRNA molecules synthesized

Answer 16

larger precurosor molecules are trimmed and chemically modified

Question 17

how does pol II know where to start

Answer 17

promoter region of DNA

Question 18

what is common in promoter regions

Answer 18

consensus DNA

Question 19

consensus sequence

Answer 19

sequence is similiar but not necessarily identifcal

Question 20

examples of consensus sequres

Answer 20

TATA box, CAAT box, GC region

Question 21

what consensus sequence is alomst always found in genes transcribed by Pol II

Answer 21

TATA box

Question 22

does pol II requre transcription factors in order to initiate transcription

Answer 22

yes

Question 23

function of TBP portion of TFIID

Answer 23

binds to TATA box, acts as a signpost

Question 24

what base pairs are easier to pull apart

Answer 24

A-T

Question 25

what are promotor regions usually rich in

Answer 25

AT pairs

Question 26

basal transcription complex

Answer 26

compex of RNA and general transcription factors

Question 27

function of basal transcription complex

Answer 27

initiates transcription at low rate

Question 28

what do GC-rich sequences bind

Answer 28

SP-1

Question 29

what does CAAT box bind

Answer 29

NF1

Question 30

function of enchanger elements

Answer 30

proteins bind here and stimulate transcription a great deal

Question 31

what is a prerequisite to transcription

Answer 31

extensive chromatin remodeling

Question 32

toxin found in death cap mushroom

Answer 32

alpha-amanitin

Question 33

how does alpha-amnitin work

Answer 33

inhbitor of Pol II

Question 34

what happens when you inhibit pol II

Answer 34

block synthesis of mRNA

Question 35

how does rigampicin work

Answer 35

potent inhibitor of RNA polyermase

Question 36

does rifampicin affect eukaryotic pol II

Answer 36

no

Question 37

what do you use rifampicin for

Answer 37

TB