Transfusion practicals

Question 1

what is Prothrombin Time (PT)

Answer 1

The PT is essentially a measure of the efficiency of the EXTRINSIC clotting system – FVII in addition to FV, FX, Prothrombin and Fibrinogen.

Question 2

what is the Activated Partial Thromboplastin Time(APTT) sensitive to

Answer 2

Heparin
Factors VIII, IX, XI, XII (I, II, V, X)
Lupus Anticoagulant
Liver disease
Vit K.

Question 3

how is fibrinogen measured

Answer 3

Measured as Clauss or derived Fibrinogen

Question 4

what is Clauss Fibrinogen based on

Answer 4

the addition of an excess of thrombin to platelet poor plasma. The fibrinogen concentration becomes the rate-limiting factor and the clotting time is a function of the fibrinogen concentration. A calibration curve shows a shortening of the clotting time with increasing fibrinogen concentration

Question 5

name some anticoagulant therapy

Answer 5

Agents inhibiting platelet function e.g aspirin.
Warfarin.
Heparin.
Thrombin Inhibitors.
FXa inhibitors.
Thrombolytic agents e.g. streptokinase
Natural Inhibitors of Coagulation.