Unit 3 - Redone Flashcards
(52 cards)
Primary Structure
Sequence of amino acids
Secondary Structure
Amino acid chains with a more complex structure
Tertiary Structure
Complex folding of polypeptide chains
Quaternary Structure
2+ polypeptide chains
Simple vs Conjugated Proteins
Conjugated proteins have a non-amino chemical associated with them, Simple proteins don’t
215nm
Peptide bonds
280nm
Aromatic rings in amino acids
Tyrosine
Tryptophan
Phenylalanine
Biuret reagent
Copper sulfate + NaOH
Do amino acids and dipeptides react with biuret? Why or why not?
No, require at least TWO peptide BONDS.
What interferants can interfere with biuret measurement?
Hemolysis and icterus
Biuret color change in the presence of protein
Blue to Violet
Total serum protein amount
6-8 g/dL
Biuret cannot be used with what specimen?
Urine, CSF
Biuret is insensitive for what kinds of proteins?
Globulins and other proteins that aren’t albumin
When would biuret’s insensitivity to other proteins be an issue?
When multiple myeloma present
Albumin measurement
Bromcresol green
Bromcresol purple
What % of plasma protein is albumin?
50%
Globulins definition
All plasma proteins NOT albumin
TP formula
Albumin + Globulins
Globulins calculation
Total protein - Albumin = Globulins
A/G Ratio
Albumin / Globulins
Albumins reference intervals
3.5-5 g/dL
Globulins reference intervals
2.5-3 g/dL
Urine protein reference intervals
1-14 mg/dL
or
< 100 mg/24hr
