Virology Quiz 3 Review HIV

Question 1

Function of MA

Answer 1

Maintains the structure of the virion and is involved in assembly at the plasma membrane

Question 2

Function of CA

Answer 2

forms the cone-or bullet shaped capsid

Question 3

Function of NC

Answer 3

Coats the viral genome

Question 4

Function of PR

Answer 4

Cleaves the Gag pre-cursor within the virion

Question 5

Function of RT

Answer 5

Converts viral genome into cDNA

Question 6

Function of IN

Answer 6

Inserts viral cDNA into host chromosomes

Question 7

Function of gp120

Answer 7

Facilitates attachment of the virion to its receptor and co-receptor

Question 8

Function of gp41

Answer 8

Induces fusion of the viral envelop with the plasma membrane or endosome

Question 9

The loss of CD4 positive T cells as a result of infection with HIV eventually causes what

Answer 9

Loss of immune competency and an increase in susceptibility to infection

Question 10

Is a single copy of the +ssRNA genome found in HIV?

Answer 10

No, it’s a double copy of +ssRNA genome

Question 11

Cellular host DNA repair enzymes are involved in what process of the HIV replication cycle?

Answer 11

Integration process

Question 12

The integration of viral DNA is reversible or irreversible

Answer 12

irreversible

Question 13

Integration results in the …

Answer 13

target site sequence of host cell DNA being duplicated / repeated on both sides of the provirus

Question 14

The viral integrase makes a staggered cut in the………at the…..

Answer 14

host cell DNA at the site of integration

Question 15

What ensures that Pol polyprotein is packaged into virus particle of retrovirus during assembly

Answer 15

the Pol protein is packaged as Gag-Pol fusion peptide

Question 16

The Gag and Pol proteins are coded for in two different reading frames in the retroviruses, what mechanism is used to produce Pol protein

Answer 16

ribosomal frameshifting

Question 17

What enzyme is responsible for converting retroviral RNA into DNA

Answer 17

Reverse Transcriptase

Question 18

What does reverse transcriptase use as the first template?

Answer 18

ssRNA viral genome

Question 19

After making the first DNA strand, what does reverse transcriptase use as a template next?

Answer 19

the newly synthesized DNA strand to make double-stranded DNA

Question 20

Does reverse transcriptase need a primer

Answer 20

Yes, tRNA primer to start synthesis

Question 21

Does reverse transcriptase depend on host cell enzymes

Answer 21

No, it relies primarily on viral RT

Question 22

What direction does reverse transcriptase read the RNA template

Answer 22

3’ to 5’

Question 23

What is the primer used to synthesize the first DNA strand with a retrovirus reverse transcriptase

Answer 23

a specific cellular tRNA

Question 24

Which genetic mutation found primarily in Caucasian populations confers an advantage against HIV infection and progression

Answer 24

CCR5Δ32