Yellow Fever Flashcards
(17 cards)
genus of yellow fever
flavivirus
yellow fever is transmitted through
mosquitoes
is there a cure or meds for yellow fever
no
is yellow fever enveloped or naked?
enveloped
how big is the yellow fever virus
40-60 nm
what is the genome of the yellow fever virus
(+) ssRNA
yellow fever virus has 1 envelope protein called
E
When humans get infected, they experience no / mild symptoms, what are those symptoms
fever / chills
body aches
nausea / vomiting
Time of infection until illness is how many days
3-6 days
The illness lasts for
5 days
1 in 7 infected get severe symptoms, what are those
high fever, jaundice, bleeding, shock + organ failure
Imagine if you didn’t know the genome had a (+) ssRNA, so you obtain a cell-free translation system, what does it contain?
eukaryotic ribosomes subunits, amino acids, initiation and elongation factors, etc is needed for mRNA to be translated to ribosomes.
what are the two controls of the cell-free translation system?
mRNA template for translation
no RNA template added
so we take the genome and put it into 3 different containers, which are
saline, DNase, RNase
Does the genome survive in the containers? What are the results
- saline: survive
- dnase: survive
- rnase: not survive
which means the genome is made of RNA since it is degraded by RNAse
When you put it into like the machine thingy idk, what comes out that makes us certain that its (+) RNA and not (-)
When the control mRNA is (+), and the result is a protein produced
When you do this experiment, what would you expect to see if the virus’ genome was (+) sense RNA?
the protein would be denatured
