1-6 Flashcards

1
Q

The essential step for Western blotting diagnosis of BSE is:

A

Treatment of the sample with proteinase K

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2
Q

Sensitized erythrocytes are:

A

Erythrocytes labelled antibody (amboceptor)

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3
Q

For testing of complement activation is NOT used:

A

Enzyme labelled antibodies specific to complement

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4
Q

Complement fixation test is used for:

A

Only for determination of antigen or antibody presence

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5
Q

In complement fixation test is positive result:

A

Inhibition of haemolysis of sensitised erythrocytes

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6
Q

Number leukocytes we may determinate by counting of cells by -

A

Microscope and Burker chamber

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7
Q

The blood in which we want determinate the number of leukocytes is -

A

Diluted in Turk solution (1:20)

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8
Q

We count the leukocytes -

A

In 50 medium squares of Burker chamber

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9
Q

For checking of viability of cells we may use -

A

Trypan blue solution

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10
Q

The live cells are cells -

A

Never stained

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11
Q

Mononuclear cells are -

A

Monocytes, macrophages and lymphocytes

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12
Q

The common characteristic of separating solution for isolation of mononuclear cells is -

A

Density (1.075-1.0077 g/ml)

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