1-6 Flashcards
The essential step for Western blotting diagnosis of BSE is:
Treatment of the sample with proteinase K
Sensitized erythrocytes are:
Erythrocytes labelled antibody (amboceptor)
For testing of complement activation is NOT used:
Enzyme labelled antibodies specific to complement
Complement fixation test is used for:
Only for determination of antigen or antibody presence
In complement fixation test is positive result:
Inhibition of haemolysis of sensitised erythrocytes
Number leukocytes we may determinate by counting of cells by -
Microscope and Burker chamber
The blood in which we want determinate the number of leukocytes is -
Diluted in Turk solution (1:20)
We count the leukocytes -
In 50 medium squares of Burker chamber
For checking of viability of cells we may use -
Trypan blue solution
The live cells are cells -
Never stained
Mononuclear cells are -
Monocytes, macrophages and lymphocytes
The common characteristic of separating solution for isolation of mononuclear cells is -
Density (1.075-1.0077 g/ml)