Immunology 5 Flashcards
GM-CSF - role in stem cell transplant?
Hit and shake bone marrow to produce more stem cells and release into bloodstream - then collect it - peripheral collection Stem cell
Glucocorticoids effect?
Neutrophilia - transient
CD4 lymphopenia
Eosinophil depletion
Inhibition of cytokines ( IL1/IFNy/TNF), prostaglandings, leukotrienes
6-Thiopurine from AZA - what it does?
Incorporate into nucleotide chain - antiproliferative effect on Lymphocytes/NK cells
Induce T cell apoptosis via Rac gene
Mycophenolate mechanism of action?
Inhibit IMDPH - so no guanine nucleotide production ( essential in T,B lymphocytes)
MTX inhibit Dihydrofolate reductase so?
So no purine (AG ) synthesis
Adenosine
Guanine
Cyclophosphamide causes hemorrhagic cystitis - how to prevent it?
MESNA hydration
IVIG mechanism of action?
Antibodies against immuno-modulatory proteins/ super-antigens + catabolism
Fc-Rn binding = recycles IgG so increases half life
How infection breaks immune tolerance?
- Anti-self T cells tolerised by self Ag BUT BY IMMATURE DC ( no B7)
- infection has Antigen - trigger by DC (has B7) - Molecular mimicry
- foreign peptide SIMILAR to self-antigen but its not completely exact same. So T cell activate and attack foreign peptide ( think done good job)
but at the same time attack self ( recognise self-antigen as foreign now)
Coeliac disease pathogenesis?
How pseudomonas fluorescence causes coeliac disease?
Gliadin ( can’t be digested) - go bind TTG
TTG deamidate glutamine. so gliadine is now DAG ( deamidated gliadin)
DAG Binds to HLA DQ2/8 - Activate PFSGDS antibodies
-trigger CD4T cells/ B cells
- Pseudomonas enter gut mucosa and anti-PFSGDS produced and drives T cells. So molecular mimicry - trigger T cells via PFSDGS antibodies
Rituximab need to bind to what to initiate complement mediated killing?
C1q
