11 Immunodiagnostics Flashcards Preview

HDM Final > 11 Immunodiagnostics > Flashcards

Flashcards in 11 Immunodiagnostics Deck (14)
Loading flashcards...
1

poor sensitivity results in

false negatives - didnt detect it when it was there

2

poor specificity results in

false positives - detected something when it wasnt there or something else made your test pop hot

3

precipitin reaction positive

forms a complex that visibly precipitates

4

agglutination reaction positive

cells/particles bound together through Ab-Ag rxn

5

Labeled Ab methods

Ab linked to label
Ab bound to Ag detected by presence of label

6

direct tests detect

Antigen

7

indirect tests detect

antibodies

8

direct flourescent Ab test will ID what

Antigen in tissue - flourescent Ab tags against Ag interact and are detected by the light

9

indirect flourescent Ab test will ID waht

Ab in patient - flourescent molecule that binds Ab added - if patient Ab is attached to Ag testing for it will bind flourescent molecule and get detected

10

precipitation will only occur with

multivalent Ag and Ab crosslinking to form large aggregates

11

ouchterlony double diffusion test can determine what and with what visible rxn

can determine if Ag contain overlapping epitopes
place Ab in the middle of different Ag- if precipitation occurs, it reacts with that Ag
can determine if they are identical, different, if there is crossreaction

12

agglutination test is typically used in

blood typing

13

direct coombs test

detects anti-Rh Ab on fetal RBCs

14

indirect coombs test

detects anti Rh Ab in mothers serum