Special Stains- Endospore

Question 1

Endospore stains are needed because

Answer 1

Endospores do not stain with Gram Staining due to their highly resistant protein coats of keratin

Question 2

What are endospores?

Answer 2

specialized , Dormant structures made by Bacillus & Clostridium bacterial genera that allow the bacterium to survive harsh conditions

Question 3

Endospore Stain Technique

Answer 3

Primary Stain (Malachite Green) (with or w/o gentle heating , rinse w/ distilled water, (removes primary stain from everything but the spores) Counterstain (Safranin) added

Question 4

Results of endospore stain include

Answer 4

Spores will be stained green, while cells will be pink

Question 5

Endospore Technique with times

Answer 5

Malachite Green (5 mins), rinse w/ distilled water, Safranin for 30 seconds, Rinse, blot dry

Question 6

Endospore staining is a differential/ structural stain that identifies

Answer 6

spores in Gram Positive bacterias

Question 7

Bacterias (that can) form endospores

Answer 7

do so when exposed to harsh conditions (like starvation)

Question 8

When vegetative cell dies,

Answer 8

endospore is released

Question 9

Because spores are highly resistant to dye, what is used to drive the primary stain of ________ to color smear?

Answer 9

heat is used to drive primary stain of malachite green