Mannitol Salt Agar test Flashcards
Purpose of Mannitol Salt Agar Test
used to identify pathogenic staphylococci species aureus vs epidermidis
What inhibits growth in Mannitol Salt agars?
The high salt concentration
If bacterial growth is seen on mannitol salt agar, this means?
The bacteria is staphylococcus
If bacterial growth is not seen on , this means?
Bacteria could not survive high salt concentrations
What should you also look for if bacterial growth is seen on mannitol salt agars?
See if the bacteria can ferment mannitol
to ferment means
to breakdown
If bacteria growth can break down mannitol, the _____ byproduct will change the dye from phenol red to what color?
acid byproduct; change from phenyl red to yellow
If bacterial growth cannot break down mannitol, the agar will be what color?
rosy pink
Can you determine if mannitol is positive or negative if there was no growth?
No; growth is needed to determine mannitol positive/negative tests. Test will be inconclusive
Example of mannitol positive bacteria
Staphylococci aureus
Example of mannitol negative bacteria
staphylococci epidermidis
Positive Mannitol tests meaning and color
it can ferment mannitol & yellow
Negative mannitol test and meaning
It cannot ferment mannitol; rosy pink
