Respiration/ Nitrate test

Question 1

removing electrons

Answer 1

oxidizing

Question 2

gaining electrons

Answer 2

reduction

Question 3

During glycolysis, glucose is _____ to pyruvate acid

Answer 3

oxidized

Question 4

metabolic processes that convert chemical energy to ATP by transferring electrons to an ETC to a proton motive force that synthesizes the ATP

Answer 4

cellular respiration

Question 5

Metabolic process in which electrons are transferred from the electron transport chain to molecular oxygen (O2) (oxygen is final electron acceptor); yields the most ATP

Answer 5

aerobic respiration

Question 6

Metabolic process in which electrons are transferred from the electron transport chain to a terminal electron acceptor other than O2. (inorganic compounds)

Answer 6

anaerobic respiration

Question 7

What lethal gas can be created during respiration? What is the enzyme most aerobic organisms produce to break it down?

Answer 7

H2O2 (hydrogen peroxide) ; catalase

Question 8

Purpose of Nitrate test

Answer 8

Looking for presence of Nitrate reductase enzyme, but since most bacteria have an enzyme NITRITE reductase, the test will look for the formation

Question 9

Nitrate test methods

Answer 9

Add Solution A and Solution B, and if it immediately turns red, it is positive for nitrite reductase; NO COLOR change = could suggest both nitrate & NITRITE reductase or neither reductases

Question 10

What should you add if there is no color change after adding both solutions in Nitrate test?

Answer 10

Zinc powder. If solution turns red after adding zinc , Nitrate reductase is NOT present. WHY? Zinc will react with the solutions that were first added to form nitrite, so color change at this point will not show if nitrate reductase is present