EMB- Eosin Methylene Blue Test

Question 1

Purpose of EMB test

Answer 1

distinguishes bacteria that FERMENT LACTOSE (E.Coli ) from other gram negative bacterias that DO NOT FERMENT LACTOSE

Question 2

IF EMB test is positive , what color will it be?

Answer 2

Green is positive for presence of E. COli

Question 3

to ferment =

Answer 3

to breakdown

Question 4

If EMB test is negative, what color will it be?

Answer 4

Negative EMB test will be pink/purple ;

Question 5

Positive EMB tests are green because?

Answer 5

The bacteria was able to ferment lactose and produce an acidic waste product thats sucks up blue dye and causes a color change

Question 6

Negative EMB tests are pink/purple because?

Answer 6

Bacteria was not able to ferment lactose. Instead it broke down proteins and produced alkaline waste product that strengthens bacteria cell wall & no dye is taken up.

Question 7

example of positive EMB test bacteria

Answer 7

E.coli

Question 8

example of negative EMB test bacteria

Answer 8

Salmonella

Question 9

EMB test can also be used to distinguish between these forms of bacteria

Answer 9

coliform vs non coliform

Question 10

EMB test encourages growth of gram negative bacteria because its dyes are ____ to gram positive bacteria??

Answer 10

toxic

Question 11

ph indicators; nutrients

Answer 11

Eosin Y & Methylene Blue; Sucrose and lactose