Thioglycollate Broth

Question 1

Purpose of thioglycolate broth ?

Answer 1

to test bacteria’s aerotolerance (how well it can live/grow in presence of molecular oxygen)

Question 2

Oxygen levels are ______ at top and ______ at bottom in fluid thyoglycolate media

Answer 2

higher at top ; lower at bottom

Question 3

bacteria grows at top of media; requires oxygen to grow

Answer 3

obligate aerobe

Question 4

bacteria grows @ bottom of media; dies in presence of O2

Answer 4

obligate anaerobe

Question 5

grows throughout test tube; prefers aerobic environment; grows best with oxygen, but also grows without it

Answer 5

facultative anaerobe

Question 6

require small amount of oxygen; grows only if small amount of oxygen is available

Answer 6

microaerophiles

Question 7

grows equally well with or with out oxygen; fermentation is their only metabolic option

Answer 7

aerotolerant