Cultures & Culture Mediums

Question 1

Nutrient preparation where microbes are grown in lab

Answer 1

culture medium

Question 2

Resulting microbial growth once microbes are introduced and grown in medium is a

Answer 2

culture

Question 3

to study microbes, we need a method to grow them , known as

Answer 3

culturing

Question 4

Culturing was a process developed by

Answer 4

Robert Koch and Fanny Hesse on the assist (agar)

Question 5

Culture media can exist in both

Answer 5

solid and liquid (broth) media

Question 6

How do microbes grow in broths?

Answer 6

Throughout the media

Question 7

Solid culture media is known as

Answer 7

agar (material derived from algae) (not metabolized by microbes)

Question 8

Microbial growth that typically happens on agar’s surface is known as

Answer 8

a colony (visible mass of cells derived from division of single cell)

Question 9

Solid media examples inclue

Answer 9

Agar plate, Agar slant, Agar deep

Question 10

Most culture mediums (nutrient prep needed for microbial growth) used in lab are

Answer 10

complex media (nutrient rich media w/ meat, peptones, veggies)

Question 11

Chemically defined medias composed of

Answer 11

pure laboratory chemicals

Question 12

As bacteria grow and divide, the broth is populated and causes

Answer 12

turbidity/ turbid look

Question 13

Presence of turbidity (cloudiness) in liquid media displays

Answer 13

growth within the medium

Question 14

Machine used to measure turbidity

Answer 14

spectrophotometer

Question 15

Streak Plate Method

Answer 15

used for isolation/ obtaining pure culture. has less surface area

Question 16

Pour plate method

Answer 16

culture + melted agar combined to see growth and primarily used to obtain CFUs/ count colonies. Has more surface area