1.1.3 separation techniques Flashcards
what can centrifuges separate
substances of different densities
where do the more dense components settle in a centrifuge separation
in the pellet
where do the less dense components settle in the centrifuge separation
supernatant
how can paper and thin later chromatography be used
separate different substances such as amino acids and sugars
what can be used for separating different substances such as amino acids and sugars
paper and thin layer chromatography
what does the speed that each solute travels along the chromatogram depend on
different solubility in the solvent used
how does affinity chromatography work
- a solid matrix or gel column is created with specific molecules bound to the matrix or gel
- soluble, target proteins in a mixture with a high affinity for these molecules become attached to them as the mixture passes down the column
- other non target molecules with a weaker affinity are washed out
how does gel electrophoresis work
charged nanomolecules move through an electric field applied to a gel matrix
how do native gels separate proteins
by shape charge and size
why do native gels separate proteins by size shape and charge
they do not denature the molecule
how does SDS-PAGE separate proteins
by size alone
how does SDS-PAGE separate proteins by size alone
it gives all the molecules an equally negative charge and denatures them
how else can proteins be separated from a mixture
through using thier isoelectric points in electrophoresis
what is an isoelectric point
the ph at which a soluble protein has no net charge and will precipitate out of solution
what will precipitate if a solution is buffered to a specific pH
the proteins with an isoelectric point of that pH
