1.1.6 aseptic technique and cell culture Flashcards
what does aseptic technique do
eliminates unwanted microbial contaminants when culturing microorganisms or cells
how is aseptic technique brought about
involves sterilisation of equipment and culture media by heat or chemical means and subsequent exclusion of microbial contaminants
how can a microbial culture be started
using an inoculum of microbial cells on an agar medium, or in a broth with suitable nutrients
what do many culture medias promote
growth of specific types of cells and microbes
how are animal cells grown
in medium containing growth factors from serum
what are growth factors
proteins that promote cell growth and proliferation. they are essential for the culture of most animal cells
how many divisions can a primary cell line do in culture
a limited number
how many divisions can a tumour cell line perform in culture
unlimited numbers
what does plating out a liquid microbial culture on solid media allow
the number of colony forming units to be counted and the density of cells in the culture to be estimated
what is often needed to achieve a suitable colony count
serial dilution
what is vital staining used for
identifying and counting viable cells
