Aileen West Nile Flashcards
What are the three structural proteins created by West Nile virus?
Capsid (C)
preM/M matrix
E: glycoprotein
What are the seven non structural proteins created by West Nile?
NS1, NS2A, NS2B, NS3, NS4A, NS4B, AND NS5
What does the Capsid (C) protein do?
Capsid protein; encloses the RNA genome, packages RNA into immature virions.
What does the prM/M protein do?
Viruses with M protein are infectious: the presence of M protein allows for the activation of proteins involved in viral entry into the cell. prM (precursor membrane) protein is present on immature virions, by further cleavage by furin to M protein, the virions become infectious
What does the E protein do?
A glycoprotein that forms the viral envelope, binds to receptors on the host cell surface in order to enter the cell.
What are all the NS proteins and what do they do?
NS1 NS1 is a cofactor for viral replication, specifically for regulation of the replication complex.[17]
NS2A NS2A has a variety of functions: it is involved in viral replication, virion assembly, and inducing host cell death.[18]
NS2B A cofactor for NS3 and together forms the NS2B-NS3 protease complex.[14] Contains transmembrane domains which bind the protease to intracellular membranes.
NS3 A serine protease that is responsible for cleaving the polyprotein to produce mature proteins; it also acts as a helicase.[12]
NS4A NS4A is a cofactor for viral replication, specifically regulates the activity of the NS3 helicase.[19]
NS4B Inhibits interferon signaling.[20]
NS5 The largest and most conserved protein of WNV, NS5 acts as a methyltransferase and a RNA polymerase, though it lacks proofreading properties.[14][21]
What type of zoonoses is West nile?
Mosquitto borne
What is the name of west nile pathogen?
West Nile Virus
What si the taxonomy of West Nile Virus?
Family: Flavivaridae
Genus: Flavivirus
Species: West Nile Virus
What serovars/clades does west Nile have?
Lineage 1: 3 clades 1a (most outbreaks), 1b (endemic in Oceania), and 1c (India)Lineage 2: Africa
What is the morphology of the West Nile Virus?
Shape: icosahedral Envelope: Host lipid membrane contians cholestrol and phospatidylserine Protein shell: glycoprotein E (180 copies, in 60 sets of 3) and membrane protein M. Capsid: capsid proteins (105AA long). RNA: ssRNA positive sense, flanked by non-coding loops Proteins: 10 total 3 structural ans 7 non structural
What is the fatality rate fo west nile?
Very low
What is the life cycle of West Nile?
Bird –> Mosquitto –> Bird
What is the infection rout of west nile?
Attachment: Glycoprotein E (2/3) binds to host cell recelptors (DSIGN, DSIGNR, and αvβ3)and aids fusion to the cell membrane. Entrance: Cholestrol plays a role in entering the host cell. Clatherin: endocytosis, endosome/endosome fusion alters pH and viral contents released Capsid: capsod proteins are the first proteins made when in the host cell. Also prevents apoptosis of the cell throught the Akt pathway. RNA: RNA is translated into a polyprotein which is then cleaved by both host and viral proteases NS2B-NS3 to produce mature proteins Replication: NS5, a RNA polymerase, forms a replication complex with other nonstructural proteins to produce an intermediary negative-sense single-stranded RNA, The negative-sense strand serves as a template for synthesis of the final positive-sense RNA Budding: During the budding process the amino particle that is on the end of the pre-M protein is released by a proteolytic cleavage process which produces a mature M protein, necessary for an infectious West Nile particle. The genomic progeny is assembled into an enveloped, icosahedral nucleocapsid
What is the history of West nile?
1937: First detected in the West Nile of Uganda
1950: Egypt: common child hood disease 1951-1954: Isreal: Naïve populations and those in nursing home infected
1996: Romania 393 cases with 17 deaths and 353 neuroinvasive illness 1999: Outbreak reached New York
