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ZOO 201 (LAB) > The Microscopy > Flashcards

The Microscopy Flashcards

1
Q

earliest form of microscope

A

crude one; relies on simple lenses and a source of light (usually the natural light of the day)

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2
Q

microscope today can produce ______

A

3D images, contrasts between parts and a higher magnification and resolution

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3
Q

two (2) principles of the microscope

A
  1. Magnification
  2. Resolution
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4
Q

this principle of the microscope is its ability to enlarge an object placed in the field of view

A

magnification

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5
Q

this principle of the microscope is the ability to clarify an object to distinguish parts from one another

A

resolution

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6
Q

magnification and resolution are important to defining the ______

A

structures of cells, tissues, and microorganisms

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7
Q

two (2) classification in which microscope can be classified

A

based on the:
1. number of lenses
2. source of illumination

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8
Q

two (2) microscope classifications based on the number of lenses

A
  1. simple microscope
  2. compound microscope
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9
Q

two (2) microscope classification based on the source of illumination

A
  1. light microscope
  2. electron microscope
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10
Q

this classification of microscope makes use of a single lens

A

simple microscope

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11
Q

classification of microscope that makes use of light

A

light microscope

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12
Q

classification of microscope that makes use of beams of electrons and with higher magnification

A

electron microscope

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13
Q

classification of microscope that makes use of two lenses

A

compound microscope

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14
Q

electron microscopes may be either:

A
  1. transmission electron microscope (TEM)
  2. scanning electron microscope (SEM)
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15
Q

electron microscope that is used to make 3D images of the specimen’s surface

A

scanning electron microscope

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16
Q

electron microscope that produces a 2D image of an ultrathin section by capturing electrons that have passed through the specimen

A

Transmission electron microscope

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17
Q

electron microscope most useful in investigating a cell’s interior

A

TEM

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18
Q

what is done for the specimens under observation to be better viewed?

A

staining

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19
Q

microscope which allows a clear view of a specimen even without staining

A

phase contrast microscope

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20
Q

microscopy wherein fluorescent dyes are used so that, when illuminated by ultraviolet dyes, would fluoresce

A

fluorescent microscopy

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21
Q

microscopy useful in tracking certain signals, genes, and structures of the body

A

fluorescent microscopoy

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22
Q

the ability of microscopes to magnify and resolve objects are made possible by this part:

A

magnifying parts

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23
Q

part that functions for enlargement and resolution of objects

A

magnifying parts

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24
Q

total magnification =

A

objective magnification x eyepiece magnification

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25
Q

magnifying power of the eyepiece

A

10x

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26
Q

five (5) tips you can follow when using the microscope in the laboratory

A
  1. Place the microscope on a level surface
  2. Check the parts before using them
  3. Handle the knobs and objectives carefully
  4. Maximize the use of the mirror
  5. Always open both eyes when looking at the specimen
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27
Q

why should one check the parts first before using the microscope?

A

there might be some parts that are missing or broken

28
Q

why should the knobs and objectives be handled carefully?

A

in order to not loosen them and also to avoid scratching their surfaces

29
Q

why should we maximize the use of the mirror?

A

this will illuminate the specimen well

30
Q

why should both eyes be open while looking at the specimen?

A

to prevent your eyes from being strained

31
Q

this microscope is a practical and convenient tool in any Biology laboratory;

it uses the visible spectrum for magnification of objects as minute as less than a millimeter in dimension, up to a small fraction of a micron

A

light compound microscope

32
Q

resolution of a light compound microscope

A

0.02 microns

33
Q

most commonly used microscope in the laboratory, passing light rays through the specimen, and bringing them to focus using system lenses

A

bright-field microscope

34
Q

two (2) parts of a light compound microscope

A
  1. optical parts
  2. mechanical parts
35
Q

four (4) parts of the microscope under the optical part

A
  1. ocular lens
  2. objective lenses
  3. abbe condenser lens
  4. mirrror
36
Q

ten (10) mechanical parts of the microscope

A
  1. draw tube
  2. dust shield
  3. revolving nosepiece
  4. stage
  5. iris diaphragm
  6. inclination joint
  7. body
  8. handle
  9. base
  10. course and fine wheels
37
Q

part through which the object is viewed

A

ocular lens

38
Q

used to collect light and has two kinds

A

mirror

39
Q

4 kinds of objective lenses

A
  1. Scanner
  2. LPO
  3. HPO
  4. Oil Immersion
40
Q

a sub-stage lens that concentrates the light rays that pass through the mounted specimen

A

abbe condenser lens

41
Q

enumerate the magnification power of the objective lenses

A
  1. scanner - 4x
  2. LPO - 10x
  3. HPO - 40x
  4. Oil immersion - 100x
42
Q

2 kinds of mirror:

A
  1. plane mirror
  2. concave mirror
43
Q

this mirror is used with the natural light

A

plane mirror

44
Q

this mirror is preferred when working with artificial or dim light

A

concave mirror

45
Q

used for holding the microscope

A

handle

46
Q

while the hand is on the handle to hold the microscope, the other hand of the user should support its ______

A

base

47
Q

it is rotated to shift from one objective lens to another

A

resolving nosepiece

48
Q

a substage centrally attached circular contraption that has overlapping strips of metal arranged akin to those of a folding fan operated with a lever to open or close;

serves to regulate the amount of light entering the condenser lens

A

iris diaphragm

49
Q

pairs of big and small knobs positioned on each side of the body of the microscope

A

course and fine wheels

50
Q

it adjusts the distance of the eyepiece from the objective lens

A

draw tube

51
Q

it is a round or forked bottom that must be laid flat on the table;

should be supported during the transport of the microscope to and from the stockroom

A

base

52
Q

a piece of metal disk fastened above the revolving nosepiece

A

dust shield

53
Q

found only in the early model, but it is conveniently used for tilting the microscope when the observer is a little tired to stoop down

A

inclination joint

54
Q

quadrangular platform with a hole at the center, is where the specimen is mounted

A

stage

55
Q

straight tube where the ocular and objective lenses are attached

A

body

56
Q

a larger microscope that can occupy a substantial part of a laboratory room

A

electron microscope

57
Q

magnification of an electron microscope can be as high as _________

A

1 milliion times

58
Q

limit of resolution of an electron microscope

A

0.2 nm

59
Q

instead of magnifying glass lenses, electron microscope uses ______ for magnification

A

series of electromagnetic fields

60
Q

electron microscope that can resolve the fine details of a cell in the interior

A

transmission electron microscope

61
Q

electron microscope that can resolve the details of the surface structure of the cell

A

scanning electron microscope

62
Q

type of bright field microscope that is usually of a low magnification;

used mainly for dissection of tiny objects, providing them a 3D image

A

stereoscopic microscope

63
Q

type of optical microscope which uses a camera that is able to project an object placed on the stage for examination in a monitor;

best used to examine histological details of tissues or the size and arrangement of bacterial cells

A

digital microscope

64
Q

microscope that uses a pinhole, where a fine beam of light (laser beam) is made to pass through the specimen, eliminating out of focus light rays

A

confocal microscope

65
Q

other term used to refer to confocal microscope

A

confocal laser scanning microscope

ZOO 201 (LAB) (14 decks)

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